ArticleScandinavian journal of gastroenterology2008
Quantitative analysis of the effect of Helicobacter pylori on the expressions of SOX2, CDX2, MUC2, MUC5AC, MUC6, TFF1, TFF2, and TFF3 mRNAs in human gastric carcinoma cells.
Article in Scandinavian journal of gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Optimization ofInternational journal of molecular sciences · 2024Article
- IFNγ-dependent silencing of TFF1 duringOpen biology · 2022Article
- Review
- TFF1 Induces Aggregation and Reduces Motility ofInternational journal of molecular sciences · 2021Article
- Structure, Function, and Therapeutic Potential of the Trefoil Factor Family in the Gastrointestinal Tract.ACS pharmacology & translational science · 2020Review
- SOX2 protein biochemistry in stemness, reprogramming, and cancer: the PI3K/AKT/SOX2 axis and beyond.Oncogene · 2020Review
- Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference toFrontiers in microbiology · 2019Article
- Bromelain inhibits the ability of colorectal cancer cells to proliferate via activation of ROS production and autophagy.PloS one · 2019Article
- Effects of the Helicobacter pylori Virulence Factor CagA and Ammonium Ion on Mucins in AGS Cells.Yonsei medical journal · 2018Article
- Transfection of theOncology letters · 2018Article
- The Hedgehog Signaling Pathway Emerges as a Pathogenic Target.Journal of developmental biology · 2017Article
- From inflammation to gastric cancer - the importance of Hedgehog/GLI signaling in Helicobacter pylori-induced chronic inflammatory and neoplastic diseases.Cell communication and signaling : CCS · 2017Review
- Trefoil Factor Peptides and Gastrointestinal Function.Annual review of physiology · 2017Review
- Gastric TFF1 Expression from Acute to ChronicFrontiers in cellular and infection microbiology · 2017Article
- Expression of CDX2 in gastric cardia adenocarcinoma and its correlation with H. pylori and cell proliferation.Oncotarget · 2016Article
- Paradoxical role of SOX2 in gastric cancer.American journal of cancer research · 2016Review
- Esophageal Helicobacter pylori colonization aggravates esophageal injury caused by reflux.World journal of gastroenterology · 2014Article
- Bromelain and N-acetylcysteine inhibit proliferation and survival of gastrointestinal cancer cells in vitro: significance of combination therapy.Journal of experimental & clinical cancer research : CR · 2014Article
- The co-expression of functional gastric proteins in dynamic gastric diseases and its clinical significance.BMC clinical pathology · 2013Article
- Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice.Digestive diseases and sciences · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveTo investigate the phenotypic characters of carcinoma cells and the response of gastric epithelial cells to Helicobacter pylori (H. pylori) infection using the gastric carcinoma cell lines. MATERIAL AND
methodsReal-time reverse transcription-polymerase chain reaction (RT-PCR) was used to assess the effect of H. pylori infection on mRNA levels of transcription factors (SOX2 and CDX2), mucin core proteins (MUC2, MUC5AC, and MUC6), and trefoil factor family peptides (TFF) (TFF1, TFF2, and TFF3) in gastric carcinoma cells (AGS, MKN45, and KATO III cells). H. pylori ATCC 43504 and its isogenic cag pathogenicity island (PAI) deleted mutant were used.
resultsThese cell lines expressed mixed gastric and intestinal phenotypes. The intestinal phenotype predominated in AGS cells and gastric phenotypes in MKN45 and KATO III cells. In all three cell lines, H. pylori infection inhibited SOX2 mRNA expression, but induced the three TFFs mRNAs. In AGS cells, H. pylori induced cag PAI-dependent mRNA expression of CDX2, MUC2, MUC5AC, and MUC6. mRNA expressions of CDX2, MUC5AC, and MUC6 were inhibited in KATO III cells, whereas MUC2 mRNA expression was unchanged. In MKN45 cells, H. pylori induced the three MUCs mRNAs but inhibited CDX2 mRNA expression.
conclusionsThis study provides a useful platform for selecting appropriate cell lines to model H. pylori-related changes in the gastric epithelium that mirror the changes seen in vivo. The outcome of H. pylori infection may reflect changes in the mucus gel layer caused by altered expression of mucins and TFF peptides.
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