ArticleMarine biotechnology (New York, N.Y.)
Preliminary analysis of length and GC content variation in the ribosomal first internal transcribed spacer (ITS1) of marine animals.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- The de novo transcriptome of the freshwater copepod Cyclops abyssorum tatricus reveals high-elevation adaptation.Scientific reports · 2026Article
- Article
- Sequence characterization of the 5S ribosomal DNA and the internal transcribed spacer (ITS) region in four European Donax species (Bivalvia: Donacidae).BMC genetics · 2018Article
- A simple and efficient total genomic DNA extraction method for individual zooplankton.SpringerPlus · 2016Article
- Molecular diagnostic markers ofSpringerPlus · 2016Article
- The 5S rDNA high dynamism in Diplodus sargus is a transposon-mediated mechanism. Comparison with other multigene families and Sparidae species.Journal of molecular evolution · 2013Article
- Evidence for 5S rDNA horizontal transfer in the toadfish Halobatrachus didactylus (Schneider, 1801) based on the analysis of three multigene families.BMC evolutionary biology · 2012Article
- Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae).Ecotoxicology (London, England) · 2012Article
- Genetic characterization of Plectorhinchus mediterraneus yields important clues about genome organization and evolution of multigene families.BMC genetics · 2012Article
- Investigation on natural diets of larval marine animals using peptide nucleic acid-directed polymerase chain reaction clamping.Marine biotechnology (New York, N.Y.) · 2011Article
- Article
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5 authors.
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Abstract
Length and guanine-cytosine (GC) content of the ribosomal first internal transcribed spacer (ITS1) were compared across a wide variety of marine animal species, and its phylogenetic utility was investigated. From a total of 773 individuals representing 599 species, we only failed to amplify the ITS1 sequence from 87 individuals by polymerase chain reaction with universal ITS1 primers. No species was found to have an ITS1 region shorter than 100 bp. In general, the ITS1 sequences of vertebrates were longer (318 to 2,318 bp) and richer in GC content (56.8% to 78%) than those of invertebrates (117 to 1,613 bp and 35.8% to 71.3%, respectively). Specifically, gelatinous animals (Cnidaria and Ctenophora) were observed to have short ITS1 sequences (118 to 422 bp) with lower GC content (35.8% to 61.7%) than the other animal taxa. Mollusca and Crustacea were diverse groups with respect to ITS1 length, ranging from 108 to 1,118 and 182 to 1,613 bp, respectively. No universal relationship between length and GC content was observed. Our data indicated that ITS1 has a limited utility for phylogenetic analysis as obtaining confident sequence alignment was often impossible between different genera of the same family and even between congeneric species.
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