ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 91 citations in OpenAlex.
- A near-complete genome assembly of onion resolves its genomic architecture, origin, and evolution.Plant communications · 2026Article
- Comparisons of mitochondrial genomes of CMS line and its nuclear and cytoplasmic donors uncover the genome rearrangement and CMS associated candidate gene in eggplant.Functional & integrative genomics · 2026Article
- Recent progress in DNA marker-assisted selection for vegetable breeding in Japan.Breeding science · 2026Article
- Phenotypic diversity and molecular characterization of male sterility based on cytotype and Ms locus variation in Indian onion germplasm.Molecular biology reports · 2025Article
- Identification of a gene coding for a pentatricopeptide repeat protein as a candidate responsible for theMolecular breeding : new strategies in plant improvement · 2025Article
- Cloning and Expression Analysis of Onion (Allium cepa L.) MADS-Box Genes and Regulation Mechanism of Cytoplasmic Male Sterility.Biochemical genetics · 2023Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Genetic Mechanisms for Hybrid Breeding in Vegetable Crops.Plants (Basel, Switzerland) · 2023Review
- Two-Step Identification of N-, S-, R- and T-Cytoplasm Types in Onion Breeding Lines Using High-Resolution Melting (HRM)-Based Markers.International journal of molecular sciences · 2023Article
- Construction of a high-resolution genetic map and identification of single nucleotide polymorphism markers relevant to flower stalk height in onion.Frontiers in plant science · 2023Article
- Molecular marker-based characterization of cytoplasm and restorer of male sterility (Ms) locus in commercially grown onions in India.Molecular biology reports · 2022Article
- Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (International journal of molecular sciences · 2021Article
- Simulation-based optimization of genomic selection scheme for accelerating genetic gain while preventing inbreeding depression in onion breeding.Breeding science · 2020Article
- Identification of a gene responsible for cytoplasmic male-sterility in onions (Allium cepa L.) using comparative analysis of mitochondrial genome sequences of two recently diverged cytoplasms.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Completion of the mitochondrial genome sequence of onion (Allium cepa L.) containing the CMS-S male-sterile cytoplasm and identification of an independent event of the ccmFCurrent genetics · 2016Article
- High-resolution tyramide-FISH mapping of markers tightly linked to the male-fertility restoration (Ms) locus of onion.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.) using a combination of bulked segregant analysis and RNA-seq.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Garlic (Allium sativum L.) fertility: transcriptome and proteome analyses provide insight into flower and pollen development.Frontiers in plant science · 2015Article
- Identification of cytoplasm types in rapeseed (Brassica napus L.) accessions by a multiplex PCR assay.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Comparison of mitochondrial and chloroplast genome segments from three onion (Allium cepa L.) cytoplasm types and identification of a trans-splicing intron of cox2.Current genetics · 2010Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel chimeric gene with a 5' end containing the nearly complete sequence of the coxI gene and a 3' end showing homology with chive orfA501 was isolated by genome walking from two cytoplasm types: CMS-S and CMS-T, both of which induce male-sterility in onion (Allium cepa L.). In addition, the normal active and variant inactive coxI genes were also isolated from onions containing the normal and CMS-S cytoplasms, respectively. The chimeric gene, designated as orf725, was nearly undetectable in normal cytoplasm, and the copy number of the normal coxI gene was significantly reduced in CMS-S cytoplasm. RT-PCR results showed that orf725 was not transcribed in normal cytoplasm. Meanwhile, the normal coxI gene, which is essential for normal mitochondrial function, was not expressed in CMS-S cytoplasm. However, both orf725 and coxI were transcribed in CMS-T cytoplasm. The expression of orf725, a putative male-sterility-inducing gene, was not affected by the presence of nuclear restorer-of-fertility gene(s) in male-fertility segregating populations originating from the cross between a male-sterile plant containing either CMS-T or CMS-S and a male-fertile plant whose genotypes of nuclear restorer gene(s) might be heterozygous. The specific stoichiometry of orf725 and coxI in the mtDNA of the three cytoplasm types was consistent among diverse germplasm. Therefore, a molecular marker based on the relative copy numbers of orf725 and coxI was designed for distinguishing among the three cytoplasm types by one simple PCR. The reliability and applicability of the molecular marker was shown by testing diverse onion germplasm.
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