ArticleThe Journal of cell biology2008
Deconstructing Survivin: comprehensive genetic analysis of Survivin function by conditional knockout in a vertebrate cell line.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
66 citing papers in PubMed, 97 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Switching off CK2-mediated activation of survivin offers new therapeutic opportunities in neuroblastoma.Experimental & molecular medicine · 2026Article
- Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes.The Journal of cell biology · 2024Article
- An unconventional regulatory circuitry involving Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes.bioRxiv : the preprint server for biology · 2024Article
- Aurora B activity is promoted by cooperation between discrete localization sites in budding yeast.Molecular biology of the cell · 2022Article
- Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments.Nature communications · 2022Article
- Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis.Scientific reports · 2021Article
- Aurora B Tension Sensing Mechanisms in the Kinetochore Ensure Accurate Chromosome Segregation.International journal of molecular sciences · 2021Review
- Localization matters: nuclear-trapped Survivin sensitizes glioblastoma cells to temozolomide by elevating cellular senescence and impairing homologous recombination.Cellular and molecular life sciences : CMLS · 2021Article
- Depletion of Survivin suppresses docetaxel-induced apoptosis in HeLa cells by facilitating mitotic slippage.Scientific reports · 2021Article
- The Borealin dimerization domain interacts with Sgo1 to drive Aurora B-mediated spindle assembly.Molecular biology of the cell · 2020Article
- The right place at the right time: Aurora B kinase localization to centromeres and kinetochores.Essays in biochemistry · 2020Review
- The whole is greater than the sum of its parts: at the intersection of order, disorder, and kinetochore function.Essays in biochemistry · 2020Review
- Establishing correct kinetochore-microtubule attachments in mitosis and meiosis.Essays in biochemistry · 2020Review
- Aurora B kinase is recruited to multiple discrete kinetochore and centromere regions in human cells.The Journal of cell biology · 2020Article
- Mitotic kinase anchoring proteins: the navigators of cell division.Cell cycle (Georgetown, Tex.) · 2020Review
- Centromere-localized Aurora B kinase is required for the fidelity of chromosome segregation.The Journal of cell biology · 2020Article
- Hec1/Ndc80 Tail Domain Function at the Kinetochore-Microtubule Interface.Frontiers in cell and developmental biology · 2020Review
- Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1.Journal of cell science · 2019Article
- Targeting the Chromosomal Passenger Complex Subunit INCENP Induces Polyploidization, Apoptosis, and Senescence in Neuroblastoma.Cancer research · 2019Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Survivin is a key cellular protein thought to function in apoptotic regulation, mitotic progression, or possibly both. In this study, we describe the isolation of two conditional knockouts of the survivin gene in chicken DT40 cells. DT40 cells lacking Survivin die in interphase after failing to complete cytokinesis. However, these cells show normal sensitivity to the chemotherapeutic agent etoposide. Expression of Survivin mutants against a null background to reassess the role of several key residues reveals that DT40 cells can grow normally if their sole Survivin is missing a widely studied cyclin-dependent kinase phosphorylation site or sites reportedly essential for binding to Smac or aurora B. Mutations in the nuclear export sequence or dimerization interface render cells temperature sensitive for growth. As an important caveat for other studies in which protein function is studied by transient transfection, three of the Survivin mutants fail to localize in the presence of the wild-type protein but do localize and indeed support life in its absence.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.