ArticleInvestigative ophthalmology & visual science2009
Enhanced inflow and outflow rates despite lower IOP in bestrophin-2-deficient mice.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 49 citations in OpenAlex.
- Neurotransmitter-bound bestrophin channel structures reveal small molecule drug targeting sites for disease treatment.Nature communications · 2024Article
- Article
- Quantitative analysis of exosomes in the aqueous humor of Korean patients with pseudoexfoliation glaucoma.Scientific reports · 2022Article
- Structures and gating mechanisms of human bestrophin anion channels.Nature communications · 2022Article
- Measurement of postmortem outflow facility using iPerfusion.Experimental eye research · 2022Article
- Structure and Function of the Bestrophin family of calcium-activated chloride channels.Channels (Austin, Tex.) · 2021Review
- Transcriptomic Network Interactions in Human Skin Treated with Topical Glucocorticoid Clobetasol Propionate.The Journal of investigative dermatology · 2019Article
- Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma.The Journal of clinical investigation · 2017Article
- Direct measurement of pressure-independent aqueous humour flow using iPerfusion.Experimental eye research · 2017Article
- Unconventional aqueous humor outflow: A review.Experimental eye research · 2017Review
- Bestrophin 1 and retinal disease.Progress in retinal and eye research · 2017Review
- Model systems for the study of steroid-induced IOP elevation.Experimental eye research · 2017Review
- Role of cyclic AMP in the eye with glaucoma.BMB reports · 2017Review
- A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum.Scientific reports · 2016Article
- Dose- and time-dependent effects of actomyosin inhibition on live mouse outflow resistance and aqueous drainage tissues.Scientific reports · 2016Article
- Measurement of Outflow Facility Using iPerfusion.PloS one · 2016Article
- Physical Factors Affecting Outflow Facility Measurements in Mice.Investigative ophthalmology & visual science · 2015Article
- Using genetic mouse models to gain insight into glaucoma: Past results and future possibilities.Experimental eye research · 2015Review
- Human aqueous humor exosomes.Experimental eye research · 2015Article
- Soluble adenylyl cyclase in the eye.Biochimica et biophysica acta · 2014Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeBestrophin-2 (Best2), a putative Cl(-) channel is expressed in the nonpigmented epithelium (NPE). Disruption of Best2 in mice results in a diminished intraocular pressure (IOP). Aqueous humor dynamics were compared in Best2(+/+) and Best2(-/-) mice, to better understand the contribution of Best2 to IOP.
methodsMeasurements of IOP, episcleral venous pressure (EVP), conventional outflow facility (C(t)), aqueous humor production (F(a)), and anterior chamber volume (V(a)) were made using anterior chamber cannulation. Conventional (F(c)) and uveoscleral outflow (F(u)), and rate of aqueous humor turnover, were calculated from measured data. The anterior chamber was examined in live mice by optical coherence tomography (OCT) and postmortem by light microscopy.
resultsIOP in Best2(-/-) mice was lower compared with Best2(+/+) littermates. EVP was unchanged. Since Best2 is expressed in NPE cells, the hypothesis was that Best2 is involved in generating aqueous flow. However, F(a) in Best2(-/-) mice was increased by approximately 73% compared with Best2(+/+) mice. This was accompanied by increases in F(c) and F(u). Aqueous humor turnover was enhanced more than twofold in Best2(-/-) mice. No evidence of developmental structural changes was noted.
conclusionsBest2 appears to antagonize the formation of aqueous humor and cause an inhibition of both F(c) and F(u), despite being expressed only in NPE cells. These data support the hypothesis that the inflow and outflow pathways communicate via soluble agents present in the aqueous humor and implicate Best2 as a critical mediator of that communication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.