ArticleThe Journal of physiology2008
Spontaneous purinergic neurotransmission in the mouse urinary bladder.
Article in The Journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Biomechanical regulation of bladder injury and repair.Nature reviews. Urology · 2026Review
- Frequency-dependent characteristics of nerve-mediated ATP and acetylcholine release from detrusor smooth muscle.Experimental physiology · 2022Article
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.Autonomic neuroscience : basic & clinical · 2021Article
- Regulation of P2X1 receptors by modulators of the cAMP effectors PKA and EPAC.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Recommendations for evaluation of bladder and bowel function in pre-clinical spinal cord injury research.The journal of spinal cord medicine · 2020Review
- Spontaneous synaptic drive in detrusor smooth muscle: computational investigation and implications for urinary bladder function.Journal of computational neuroscience · 2019Article
- ATP transients accompany spontaneous contractions in isolated guinea-pig detrusor smooth muscle.Experimental physiology · 2019Article
- Electrophysiology of Syncytial Smooth Muscle.Journal of experimental neuroscience · 2019Review
- Article
- Investigation of the Syncytial Nature of Detrusor Smooth Muscle as a Determinant of Action Potential Shape.Frontiers in physiology · 2018Article
- A biophysically constrained computational model of the action potential of mouse urinary bladder smooth muscle.PloS one · 2018Article
- A Method for the Analysis of AP Foot Convexity: Insights into Smooth Muscle Biophysics.Frontiers in bioengineering and biotechnology · 2017Article
- Transient contractions of urinary bladder smooth muscle are drivers of afferent nerve activity during filling.The Journal of general physiology · 2016Article
- A computational model of urinary bladder smooth muscle syncytium : validation and investigation of electrical properties.Journal of computational neuroscience · 2015Article
- The purinergic neurotransmitter revisited: a single substance or multiple players?Pharmacology & therapeutics · 2014Review
- Purinergic signalling in the urinary tract in health and disease.Purinergic signalling · 2014Review
- L-type Ca2+ channel sparklets revealed by TIRF microscopy in mouse urinary bladder smooth muscle.PloS one · 2014Article
- Article
- Signalling to contractile proteins by muscarinic and purinergic pathways in neurally stimulated bladder smooth muscle.The Journal of physiology · 2012Article
- Modulation of purinergic neuromuscular transmission by phorbol dibutyrate is independent of protein kinase C in murine urinary bladder.The Journal of pharmacology and experimental therapeutics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
Spontaneous purinergic neurotransmission was characterized in the mouse urinary bladder, a model for the pathological or ageing human bladder. Intracellular electrophysiological recording from smooth muscle cells of the detrusor muscle revealed spontaneous depolarizations, distinguishable from spontaneous action potentials (sAPs) by their amplitude (< 40 mV) and insensitivity to the L-type Ca(2+) channel blocker nifedipine (1 microm) (100 +/- 29%). Spontaneous depolarizations were abolished by the P2X(1) receptor antagonist NF449 (10 microm) (frequency 8.5 +/- 8.5% of controls), insensitive to the muscarinic acetylcholine receptor antagonist atropine (1 microm) (103.4 +/- 3.0%), and became more frequent in latrotoxin (LTX; 1 nm) (438 +/- 95%), suggesting that they are spontaneous excitatory junction potentials (sEJPs). Such sEJPs were correlated, in amplitude and timing, with focal Ca(2+) transients in smooth muscle cells (measured using confocal microscopy), suggesting a common origin: ATP binding to P2X(1) receptors. sAPs were abolished by NF449, insensitive to atropine (126 +/- 39%) and increased in frequency by LTX (930 +/- 450%) suggesting a neurogenic, purinergic origin, in common with sEJPs. By comparing the kinetics of sAPs and sEJPs, we demonstrated that sAPs occur when sufficient cation influx through P2X(1) receptors triggers L-type Ca(2+) channels; the first peak of the differentiated rising phase of depolarizations - attributed to the influx of cations through the P2X(1) receptor - is of larger amplitude for sAPs (2248 mV s(-1)) than sEJPs (439 mV s(-1)). Surprisingly, sAPs in the mouse urinary bladder, unlike those from other species, are triggered by stochastic ATP release from parasympathetic nerve terminals rather than being myogenic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.