ArticleWorld journal of gastroenterology2008
Facilitating effects of berberine on rat pancreatic islets through modulating hepatic nuclear factor 4 alpha expression and glucokinase activity.
Article in World journal of gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 59 citations in OpenAlex.
- Berberine is an insulin secretagogue targeting the KCNH6 potassium channel.Nature communications · 2021Trial
- Berberine: A Rising Star in the Management of Type 2 Diabetes-Novel Insights into Its Anti-Inflammatory, Metabolic, and Epigenetic Mechanisms.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Lannea edulis lowers blood glucose by modulating absorption, utilization, and pancreatic function in diabetic rats.Frontiers in pharmacology · 2025Article
- Protective effects of berberine-loaded chitosan/solid lipid nanoparticles in streptozotocin-induced gestational diabetes mellitus rats.Experimental biology and medicine (Maywood, N.J.) · 2025Article
- A Review ofMolecules (Basel, Switzerland) · 2023Review
- Role of potential bioactive metabolites from traditional Chinese medicine for type 2 diabetes mellitus: An overview.Frontiers in pharmacology · 2022Review
- Dietary berberine can ameliorate glucose metabolism disorder of Megalobrama amblycephala exposed to a high-carbohydrate diet.Fish physiology and biochemistry · 2021Article
- Berberine Potentiates Insulin Secretion and Prevents β-cell Dysfunction Through the miR-204/SIRT1 Signaling Pathway.Frontiers in pharmacology · 2021Article
- Pharmacokinetics and Pharmacological Activities of Berberine in Diabetes Mellitus Treatment.Evidence-based complementary and alternative medicine : eCAM · 2021Review
- Coptidis Rhizoma: a comprehensive review of its traditional uses, botany, phytochemistry, pharmacology and toxicology.Pharmaceutical biology · 2019Review
- Berberine Induces Cell Apoptosis through Cytochrome C/Apoptotic Protease-Activating Factor 1/Caspase-3 and Apoptosis Inducing Factor Pathway in Mouse Insulinoma Cells.Chinese journal of integrative medicine · 2019Article
- Amorphous solid dispersion of Berberine mitigates apoptosis via iPLAInternational journal of biological sciences · 2019Article
- Gastric cancer biomarkers; A systems biology approach.Biochemistry and biophysics reports · 2018Article
- Barberry in the treatment of obesity and metabolic syndrome: possible mechanisms of action.Diabetes, metabolic syndrome and obesity : targets and therapy · 2018Review
- Berberine Attenuated Proliferation, Invasion and Migration by Targeting the AMPK/HNF4α/WNT5A Pathway in Gastric Carcinoma.Frontiers in pharmacology · 2018Article
- Glucose plus metformin compared with glucose alone on β-cell function in mouse pancreatic islets.Biomedical reports · 2015Article
- Application of berberine on treating type 2 diabetes mellitus.International journal of endocrinology · 2015Review
- An overview on antidiabetic medicinal plants having insulin mimetic property.Asian Pacific journal of tropical biomedicine · 2012Review
- Berberine protects against high fat diet-induced dysfunction in muscle mitochondria by inducing SIRT1-dependent mitochondrial biogenesis.Biochimica et biophysica acta · 2012Article
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Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo observe the effect of berberine on insulin secretion in rat pancreatic islets and to explore its possible molecular mechanism.
methodsPrimary rat islets were isolated from male Sprague-Dawley rats by collagenase digestion and treated with different concentrations (1, 3, 10 and 30 micromol/L) of berberine or 1 micromol/L Glibenclamide (GB) for 24 h. Glucose-stimulated insulin secretion (GSIS) assay was conducted and insulin was determined by radioimmunoassay. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate cytotoxicity. The mRNA level of hepatic nuclear factor 4 alpha (HNF4alpha) was determined by reverse transcription polymerase chain reaction (RT-PCR). Indirect immunofluorescence staining and Western blot analysis were employed to detect protein expression of HNF4alpha in the islets. Glucokinase (GK) activity was measured by spectrophotometric method.
resultsBerberine enhanced GSIS rather than basal insulin secretion dose-dependently in rat islets and showed no significant cytotoxicity on islet cells at the concentration of 10 mumol/L. Both mRNA and protein expressions of HNF4alpha were up-regulated by berberine in a dose-dependent manner, and GK activity was also increased accordingly. However, GB demonstrated no regulatory effects on HNF4alpha expression or GK activity.
conclusionBerberine can enhance GSIS in rat islets, and probably exerts the insulinotropic effect via a pathway involving HNF4alpha and GK, which is distinct from sulphonylureas (SUs).
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