Evidence map›Paper›PMID 18931300›Full record

ArticleApplied and environmental microbiology2008

Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356.

Mariano Prado Acosta, María Mercedes Palomino, Mariana C Allievi, Carmen Sanchez Rivas, Sandra M Ruzal

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
3.7field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 47 citations in OpenAlex.

  1. The activity of cell-free supernatant ofFrontiers in cellular and infection microbiology · 2025
    Article
  2. Consortium ofAntibiotics (Basel, Switzerland) · 2024
    Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Immunostimulation byThe Journal of biological chemistry · 2020
    Article
  9. Article
  10. Article
  11. Surface (S) Layer Proteins ofFrontiers in microbiology · 2019
    Article
  12. Article
  13. Role of S-layer proteins in bacteria.World journal of microbiology & biotechnology · 2015
    Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection.Biochemical and biophysical research communications · 2012
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Mariano Prado AcostaDepartamento de Química Biológica, Facultad Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
María Mercedes Palomino
Mariana C Allievi
Carmen Sanchez Rivas
Sandra M Ruzal
University of Buenos Aires · ARFundación Ciencias Exactas y Naturales · AR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in Bacillus subtilis resulted in the functional verification of the enzymatic activity.

Indexed as

Amino Acid SequenceBacillus subtilisCell WallCloning, MolecularLactobacillus acidophilusMembrane GlycoproteinsMolecular Sequence DataN-Acetylmuramoyl-L-alanine AmidaseSequence AlignmentMembrane GlycoproteinsN-Acetylmuramoyl-L-alanine AmidaseS-layer proteins

Identifiers

PMID18931300
PMCPMC2607185
OpenAlexW2161976205

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.