ArticleApplied and environmental microbiology2008
Multilocus genotyping assays for single nucleotide polymorphism-based subtyping of Listeria monocytogenes isolates.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 160 citations in OpenAlex.
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- Is the persistence ofApplied and environmental microbiology · 2024Article
- Current methodologies available to evaluate the virulence potential amongFrontiers in microbiology · 2024Review
- Characterisation of the growth behaviour of Listeria monocytogenes in Listeria synthetic media.Environmental microbiology reports · 2023Article
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- Horizontal Gene Transfer and Loss of Serotype-Specific Genes in Listeria monocytogenes Can Lead to Incorrect Serotype Designations with a Commonly-Employed Molecular Serotyping Scheme.Microbiology spectrum · 2023Article
- Genomic and pathogenicity islands ofFrontiers in molecular biosciences · 2023Review
- Retrospective Investigation of the Whole Genome of the HypovirulentMicroorganisms · 2022Article
- Characterization of Listeria monocytogenes isolated from wildlife in central New York.Veterinary medicine and science · 2022Article
- Distribution and Characteristics ofMicroorganisms · 2022Article
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- Different Shades ofFrontiers in microbiology · 2021Article
- Genetic diversity, virulence and distribution of antimicrobial resistance amongIranian journal of veterinary research · 2021Article
- Lineage-specific evolution and gene flow in Listeria monocytogenes are independent of bacteriophages.Environmental microbiology · 2020Article
- Large-Scale Comparison of Toxin and Antitoxins inToxins · 2020Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is responsible for serious invasive illness associated with consumption of contaminated food and places a significant burden on public health and the agricultural economy. We recently developed a multilocus genotyping (MLGT) assay for high-throughput subtype determination of L. monocytogenes lineage I isolates based on interrogation of single nucleotide polymorphisms (SNPs) via multiplexed primer extension reactions. Here we report the development and validation of two additional MLGT assays that address the need for comprehensive DNA sequence-based subtyping of L. monocytogenes. The first of these novel MLGT assays targeted variation segregating within lineage II, while the second assay combined probes for lineage III strains with probes for strains representing a recently characterized fourth evolutionary lineage (IV) of L. monocytogenes. These assays were based on nucleotide variation identified in >3.8 Mb of comparative DNA sequence and consisted of 115 total probes that differentiated 93% of the 100 haplotypes defined by the multilocus sequence data. MLGT reproducibly typed the 173 isolates used in SNP discovery, and the 10,448 genotypes derived from MLGT analysis of these isolates were consistent with DNA sequence data. Application of the MLGT assays to assess subtype prevalence among isolates from ready-to-eat foods and food-processing facilities indicated a low frequency (6.3%) of epidemic clone subtypes and a substantial population of isolates (>30%) harboring mutations in inlA associated with attenuated virulence in cell culture and animal models. These mutations were restricted to serogroup 1/2 isolates, which may explain the overrepresentation of serotype 4b isolates in human listeriosis cases.
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