ArticleApplied and environmental microbiology2008
Development and application of flow-cytometric techniques for analyzing and sorting endospore-forming clostridia.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 84 citations in OpenAlex.
- Xylanase production by Thermobacillus xylanilyticus is impaired by population diversification but can be mitigated based on the management of cheating behavior.Microbial cell factories · 2022Article
- Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021Review
- Changes in efflux pump activity of Clostridium beijerinckii throughout ABE fermentation.Applied microbiology and biotechnology · 2021Article
- Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins.Applied and environmental microbiology · 2020Article
- Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives.Journal of industrial microbiology & biotechnology · 2020Review
- Article
- Degeneration of industrial bacteria caused by genetic instability.World journal of microbiology & biotechnology · 2020Review
- Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges.World journal of microbiology & biotechnology · 2020Review
- A Strongly Fluorescing Anaerobic Reporter and Protein-Tagging System forApplied and environmental microbiology · 2019Article
- Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating.PloS one · 2019Article
- Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq.BMC genomics · 2018Article
- Flow cytometry analysis ofBiotechnology for biofuels · 2018Article
- Article
- Adenine Addition Restores Cell Viability and Butanol Production in Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) Cultivated at 37°C.Applied and environmental microbiology · 2017Article
- Mathematical modelling of clostridial acetone-butanol-ethanol fermentation.Applied microbiology and biotechnology · 2017Review
- Investigation of availability of a high throughput screening method for predicting butanol solvent -producing ability of Clostridium beijerinckii.BMC microbiology · 2016Article
- Transcription factor-based biosensors in biotechnology: current state and future prospects.Applied microbiology and biotechnology · 2016Review
- Integrated, systems metabolic picture of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The Clostridium sporulation programs: diversity and preservation of endospore differentiation.Microbiology and molecular biology reviews : MMBR · 2015Review
- σK of Clostridium acetobutylicum is the first known sporulation-specific sigma factor with two developmentally separated roles, one early and one late in sporulation.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
The study of microbial heterogeneity at the single-cell level is a rapidly growing area of research in microbiology and biotechnology due to its significance in pathogenesis, environmental biology, and industrial biotechnologies. However, the tools available for efficiently and precisely probing such heterogeneity are limited for most bacteria. Here we describe the development and application of flow-cytometric (FC) and fluorescence-assisted cell-sorting techniques for the study of endospore-forming bacteria. We show that by combining FC light scattering (LS) with nucleic acid staining, we can discriminate, quantify, and enrich all sporulation-associated morphologies exhibited by the endospore-forming anaerobe Clostridium acetobutylicum. Using FC LS analysis, we quantitatively show that clostridial cultures commonly perform multiple rounds of sporulation and that sporulation is induced earlier by the overexpression of Spo0A, the master regulator of endospore formers. To further demonstrate the power of our approach, we employed FC LS analysis to generate compelling evidence to challenge the long-accepted view in the field that the clostridial cell form is the solvent-forming phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.