ArticleBiophysical journal2008
Vesicle diffusion close to a membrane: intermembrane interactions measured with fluorescence correlation spectroscopy.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Unraveling the hidden temporal range of fast βCommunications biology · 2022Article
- Eco-evolutionary feedbacks mediated by bacterial membrane vesicles.FEMS microbiology reviews · 2021Review
- In vitro single vesicle fusion assays based on pore-spanning membranes: merits and drawbacks.European biophysics journal : EBJ · 2021Review
- Differential Diffusional Properties in Loose and Tight Docking Prior to Membrane Fusion.Biophysical journal · 2020Article
- Understanding biochemical processes in the presence of sub-diffusive behavior of biomolecules in solution and living cells.Biophysical reviews · 2019Review
- Sensitivity and Robustness of Spatially Dependent Thrombin Generation and Fibrin Clot Propagation.Biophysical journal · 2018Article
- A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion.Journal of the American Chemical Society · 2016Article
- Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM).Frontiers in neuroanatomy · 2015Article
- Probing the stochastic, motor-driven properties of the cytoplasm using force spectrum microscopy.Cell · 2014Article
- Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations.The journal of physical chemistry. B · 2011Article
- Electrostatic interactions of fluorescent molecules with dielectric interfaces studied by total internal reflection fluorescence correlation spectroscopy.International journal of molecular sciences · 2010Article
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
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Authors and funding
2 authors.
Funding
Abstract
The protein machinery controlling membrane fusion (or fission) has been well studied; however, the role of vesicle diffusion near membranes in these critical processes remains unclear. We experimentally and theoretically investigated the dynamics of small vesicles (approximately 50 nm in diameter) that are diffusing near supported planar bilayers acting as "target" membranes. Using total internal reflection-fluorescence correlation spectroscopy, we examined the validity of theoretical analyses of vesicle-membrane interactions. Vesicles were hindered by hydrodynamic drag as a function of their proximity to the planar bilayer. The population distributions and diffusion kinetics of the vesicles were further affected by changing the ionic strength and pH of the buffer, as well as the lipid composition of the planar membrane. Effective surface charges on neutral bilayers were also analyzed by comparing experimental and theoretical data, and we show the possibility that vesicle dynamics can be modified by surface charge redistribution of the planar bilayer. Based on these results, we hypothesize that the dynamics of small vesicles, diffusing close to biomembranes, may be spatially restricted by altering local physiological conditions (e.g., salt concentration, lipid composition, and pH), which may represent an additional mechanism for controlling fusion (or fission) dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.