ArticleBiophysical journal2008
Twirling of actin by myosins II and V observed via polarized TIRF in a modified gliding assay.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Force-Induced Ankle Opening Reveals Mechanical Stabilization of the Ankle of Human β-Cardiac Myosin.ACS nano · 2026Article
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- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- KnotResolver: tracking self-intersecting filaments in microscopy using directed graphs.Bioinformatics (Oxford, England) · 2024Article
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Membrane-bound myosin IC drives the chiral rotation of the gliding actin filament around its longitudinal axis.Scientific reports · 2023Article
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- Twist response of actin filaments.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Formation of cellular close-ended tunneling nanotubes through mechanical deformation.Science advances · 2022Article
- CYK-1/Formin activation in cortical RhoA signaling centers promotes organismal left-right symmetry breaking.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Nanoscopic changes in the lattice structure of striated muscle sarcomeres involved in the mechanism of spontaneous oscillatory contraction (SPOC).Scientific reports · 2020Article
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- Optimal sidestepping of intraflagellar transport kinesins regulates structure and function of sensory cilia.The EMBO journal · 2020Article
- Mechanical stress induces a scalable switch in cortical flow polarization during cytokinesis.Journal of cell science · 2019Article
- Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy.International journal of molecular sciences · 2019Review
- Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro.Nature communications · 2017Article
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- Working stroke of the kinesin-14, ncd, comprises two substeps of different direction.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Myosin S2 origins track evolution of strong binding on actin by azimuthal rolling of motor domain.Biophysical journal · 2015Article
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Authors and funding
4 authors.
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Abstract
The force generated between actin and myosin acts predominantly along the direction of the actin filament, resulting in relative sliding of the thick and thin filaments in muscle or transport of myosin cargos along actin tracks. Previous studies have also detected lateral forces or torques that are generated between actin and myosin, but the origin and biological role of these sideways forces is not known. Here we adapt an actin gliding filament assay to measure the rotation of an actin filament about its axis ("twirling") as it is translocated by myosin. We quantify the rotation by determining the orientation of sparsely incorporated rhodamine-labeled actin monomers, using polarized total internal reflection microscopy. To determine the handedness of the filament rotation, linear incident polarizations in between the standard s- and p-polarizations were generated, decreasing the ambiguity of our probe orientation measurement fourfold. We found that whole myosin II and myosin V both twirl actin with a relatively long (approximately 1 microm), left-handed pitch that is insensitive to myosin concentration, filament length, and filament velocity.
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