ArticleBiophysical journal2009
Spatiotemporal analysis of cell response to a rigidity gradient: a quantitative study using multiple optical tweezers.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration.Burns & trauma · 2023Article
- Simultaneous assessment of radial and axial myocyte mechanics by combining atomic force microscopy and carbon fibre techniques.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Adipose matrix complex: a high-rigidity collagen-rich adipose-derived material for fat grafting.Aging · 2021Article
- Fluid shear stress coupled with narrow constrictions induce cell type-dependent morphological and molecular changes in SK-BR-3 and MDA-MB-231 cells.Scientific reports · 2020Article
- Bile canaliculi remodeling activates YAP via the actin cytoskeleton during liver regeneration.Molecular systems biology · 2020Article
- Mechanical checkpoint for persistent cell polarization in adhesion-naive fibroblasts.Biophysical journal · 2014Article
- Spatial control of adult stem cell fate using nanotopographic cues.Biomaterials · 2014Article
- Mechanotransduction of fluid stresses governs 3D cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Stiffening hydrogels to probe short- and long-term cellular responses to dynamic mechanics.Nature communications · 2012Article
- Rigidity sensing explained by active matter theory.Biophysical journal · 2011Article
- Force generation upon T cell receptor engagement.PloS one · 2011Article
- Article
- Adhesion dynamics and durotaxis in migrating cells.Physical biology · 2011Article
- The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF.Scientific reports · 2011Article
- Mechanics and contraction dynamics of single platelets and implications for clot stiffening.Nature materials · 2011Article
- Microfabricated substrates as a tool to study cell mechanotransduction.Medical & biological engineering & computing · 2010Review
- Real-time single-cell response to stiffness.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Vascular smooth muscle cell durotaxis depends on substrate stiffness gradient strength.Biophysical journal · 2009Article
- Multiplexed force measurements on live cells with holographic optical tweezers.Optics express · 2009Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigate the dynamic response of single cells to weak and local rigidities, applied at controlled adhesion sites. Using multiple latex beads functionalized with fibronectin, and each trapped in its own optical trap, we study the reaction in real time of single 3T3 fibroblast cells to asymmetrical tensions in the tens of pN x microm(-1) range. We show that the cell feels a rigidity gradient even at this low range of tension, and over time develops an adapted change in the force exerted on each adhesion site. The rate at which force increases is proportional to trap stiffness. Actomyosin recruitment is regulated in space and time along the rigidity gradient, resulting in a linear relationship between the amount of recruited actin and the force developed independently in trap stiffness. This time-regulated actomyosin behavior sustains a constant and rigidity-independent velocity of beads inside the traps. Our results show that the strengthening of extracellular matrix-cytoskeleton linkages along a rigidity gradient is regulated by controlling adhesion area and actomyosin recruitment, to maintain a constant deformation of the extracellular matrix.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.