ArticlePlant physiology2008
Transcriptomic and reverse genetic analyses of branched-chain fatty acid and acyl sugar production in Solanum pennellii and Nicotiana benthamiana.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 113 citations in OpenAlex.
- Structurally diverse acylsugars on wild tomatillo fruit surfaces.Horticulture research · 2026Article
- Evolutionary hijacking of fatty acid biosynthesis drives insecticidal acylsugar diversity in tomato.Nature communications · 2026Article
- Trichome formation inFrontiers in plant science · 2026Article
- A century of studying plant secondary metabolism-From "what?" to "where, how, and why?"Plant physiology · 2024Review
- Tomato root specialized metabolites evolved through gene duplication and regulatory divergence within a biosynthetic gene cluster.Science advances · 2024Article
- Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components.Plant physiology · 2023Article
- Blurred lines: Primary metabolic machinery coopted for specialized metabolism in tomato trichomes.Plant physiology · 2023Article
- Natural variation meets synthetic biology: Promiscuous trichome-expressed acyltransferases from Nicotiana.Plant physiology · 2022Article
- Article
- Acylsugars protect Nicotiana benthamiana against insect herbivory and desiccation.Plant molecular biology · 2022Article
- Pennelliiside D, a New Acyl Glucose fromMolecules (Basel, Switzerland) · 2022Article
- An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome.Plant methods · 2022Article
- Methyl jasmonate treatment, aphid resistance assay, and transcriptomic analysis revealed different herbivore defensive roles between tobacco glandular and non-glandular trichomes.Plant cell reports · 2022Article
- Key Enzymes Involved in the Synthesis of Hops Phytochemical Compounds: From Structure, Functions to Applications.International journal of molecular sciences · 2021Review
- An Integrated Analytical Approach Reveals Trichome Acylsugar Metabolite Diversity in the Wild TomatoMetabolites · 2020Article
- Article
- Patterns of inheritance of acylsugar acyl groups in selected interspecific hybrids of genus Nicotiana.Journal of plant research · 2020Article
- Candidate Gene Networks for Acylsugar Metabolism and Plant Defense in Wild TomatoThe Plant cell · 2020Article
- QTL mapping of insect resistance components of Solanum galapagense.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acyl sugars containing branched-chain fatty acids (BCFAs) are exuded by glandular trichomes of many species in Solanaceae, having an important defensive role against insects. From isotope-feeding studies, two modes of BCFA elongation have been proposed: (1) fatty acid synthase-mediated two-carbon elongation in the high acyl sugar-producing tomato species Solanum pennellii and Datura metel; and (2) alpha-keto acid elongation-mediated one-carbon increments in several tobacco (Nicotiana) species and a Petunia species. To investigate the molecular mechanisms underlying BCFAs and acyl sugar production in trichomes, we have taken a comparative genomic approach to identify critical enzymatic steps followed by gene silencing and metabolite analysis in S. pennellii and Nicotiana benthamiana. Our study verified the existence of distinct mechanisms of acyl sugar synthesis in Solanaceae. From microarray analyses, genes associated with alpha-keto acid elongation were found to be among the most strongly expressed in N. benthamiana trichomes only, supporting this model in tobacco species. Genes encoding components of the branched-chain keto-acid dehydrogenase complex were expressed at particularly high levels in trichomes of both species, and we show using virus-induced gene silencing that they are required for BCFA production in both cases and for acyl sugar synthesis in N. benthamiana. Functional analysis by down-regulation of specific KAS I genes and cerulenin inhibition indicated the involvement of the fatty acid synthase complex in BCFA production in S. pennellii. In summary, our study highlights both conserved and divergent mechanisms in the production of important defense compounds in Solanaceae and defines potential targets for engineering acyl sugar production in plants for improved pest tolerance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.