ArticlePlant physiology2008
A genome-wide and metabolic analysis determined the adaptive response of Arabidopsis cells to folate depletion induced by methotrexate.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Wavelength-Specific Transcriptomic Responses ofInsects · 2026Article
- Fluorescence spectroscopy combined with molecular docking to study the interaction between folic acid derivatives from wheat grains and bovine trypsin.Scientific reports · 2025Article
- Development of an improved construct for spectinomycin selection in plant transformation.BMC research notes · 2025Article
- Folate depletion impact on the cell cycle results in restricted primary root growth in Arabidopsis.Plant molecular biology · 2025Article
- Article
- TOR coordinates nucleotide availability with ribosome biogenesis in plants.The Plant cell · 2021Article
- Interactions between metabolism and chromatin in plant models.Molecular metabolism · 2020Review
- Dihydrofolate Reductase/Thymidylate Synthase Fine-Tunes the Folate Status and Controls Redox Homeostasis in Plants.The Plant cell · 2017Article
- Folates in Plants: Research Advances and Progress in Crop Biofortification.Frontiers in chemistry · 2017Review
- Does Abiotic Stress Cause Functional B Vitamin Deficiency in Plants?Plant physiology · 2016Review
- MTHFD1 controls DNA methylation in Arabidopsis.Nature communications · 2016Article
- C1 Metabolism Inhibition and Nitrogen Deprivation Trigger Triacylglycerol Accumulation inFrontiers in plant science · 2016Article
- Loss of function of folylpolyglutamate synthetase 1 reduces lignin content and improves cell wall digestibility in Arabidopsis.Biotechnology for biofuels · 2015Article
- Chemical probes in plant epigenetics studies.Plant signaling & behavior · 2013Review
- A somaclonal line SE7 of finger millet (Eleusine coracana) exhibits modified cytokinin homeostasis and increased grain yield.Journal of experimental botany · 2012Article
- Inhibition of p-aminobenzoate and folate syntheses in plants and apicomplexan parasites by natural product rubreserine.The Journal of biological chemistry · 2012Article
- Sulfamethazine suppresses epigenetic silencing in Arabidopsis by impairing folate synthesis.The Plant cell · 2012Article
- The plastidial folylpolyglutamate synthetase and root apical meristem maintenance.Plant signaling & behavior · 2011Article
- The folylpolyglutamate synthetase plastidial isoform is required for postembryonic root development in Arabidopsis.Plant physiology · 2011Article
- Arabidopsis putative selenium-binding protein1 expression is tightly linked to cellular sulfur demand and can reduce sensitivity to stresses requiring glutathione for tolerance.Plant physiology · 2009Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Control of folate homeostasis is essential to sustain the demand for one-carbon (C1) units that are necessary for major biological functions, including nucleotide synthesis and methylation reactions. In this study, we analyzed the genome-wide and metabolic adaptive response of Arabidopsis (Arabidopsis thaliana) cells to folate depletion induced by the antifolate methotrexate. Drug treatment induced a response typical to xenobiotic stress and important changes in folate content and composition. This resulted in a reduction of cell division and primary energy metabolism that was likely associated with perturbation of nucleotide homeostasis. Through a modification of serine metabolism, folate depletion also induced O-acetylserine accumulation and mimicked sulfur deficiency response. The major adaptive response to folate limitation concerned the composition of the folate pool rather than the intracellular level of cofactors. Thus, no significant change in the expression of genes involved in cofactor synthesis, degradation, or trafficking was observed. However, changes in the distribution of C1 derivative pools and increased expression levels for transcripts coding enzymes manipulating C1 moieties in plastids suggested a reorientation of C1 units toward the synthesis of purine and thymidylate. Also, no genomic or metabolic adaptation was built up to counterbalance the major impairment of the methyl index, which controls the efficiency of methylation reactions in the cell. Together, these data suggested that the metabolic priority of Arabidopsis cells in response to folate limitation was to shuttle the available folate derivatives to the synthesis of nucleotides at the expense of methylation reactions.
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