ArticleJournal of bacteriology2008
Amino acid substitutions in transmembrane domains 9 and 10 of GerVB that affect the germination properties of Bacillus megaterium spores.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Early activation of bioenergetic metabolism powers bacterial spore germination.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genomic identification of germinant receptors and phenotypic response to germinants inCurrent research in microbial sciences · 2025Article
- Genetic Suppression Meets Structure Prediction: Probing a Spore Germination Receptor Complex.Journal of bacteriology · 2022Article
- Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.Journal of bacteriology · 2019Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Spore Heat Activation Requirements and Germination Responses Correlate with Sequences of Germinant Receptors and with the Presence of a SpecificApplied and environmental microbiology · 2017Article
- Analysis of Germination Capacity and Germinant Receptor (Sub)clusters of Genome-Sequenced Bacillus cereus Environmental Isolates and Model Strains.Applied and environmental microbiology · 2017Article
- Structure-function analysis of the Bacillus megaterium GerUD spore germinant receptor protein.FEMS microbiology letters · 2015Article
- Bacillus thermoamylovorans Spores with Very-High-Level Heat Resistance Germinate Poorly in Rich Medium despite the Presence of ger Clusters but Efficiently upon Exposure to Calcium-Dipicolinic Acid.Applied and environmental microbiology · 2015Article
- Article
- L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences.BMC microbiology · 2014Article
- Investigating the functional hierarchy of Bacillus megaterium PV361 spore germinant receptors.Journal of bacteriology · 2013Article
- Structure-based functional studies of the effects of amino acid substitutions in GerBC, the C subunit of the Bacillus subtilis GerB spore germinant receptor.Journal of bacteriology · 2011Article
- Crystal structure of the GerBC component of a Bacillus subtilis spore germinant receptor.Journal of molecular biology · 2010Article
- Identification of a receptor subunit and putative ligand-binding residues involved in the Bacillus megaterium QM B1551 spore germination response to glucose.Journal of bacteriology · 2010Article
- Factors affecting variability in time between addition of nutrient germinants and rapid dipicolinic acid release during germination of spores of Bacillus species.Journal of bacteriology · 2010Article
- The Ger receptor family from sporulating bacteria.Current issues in molecular biology · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular basis for differences in germinant recognition of Bacillus megaterium QM B1551 spores containing the GerVB and/or GerUB receptor proteins has been examined by site-directed mutagenesis and the construction of cross-homologue chimeras. Focusing on nonconserved residues predicted to reside in transmembrane domains 9 and 10, we demonstrate that GerVB residues Ser319 and Leu345 are of particular importance in defining the specificity and apparent affinity of the receptor for germinants. Kinetic analyses of mutants with different amino acid substitutions at these positions indicate that Ser319 and Leu345 are not involved directly in the binding of germinants, but probably reside in regions of the receptor where structural perturbations can affect the conformation of, or access to, germinant binding sites. Position 345 is also shown to be of importance in GerUB, where the F345A mutation severely impairs receptor function. Functionality is restored in the GerUB Ala345 background by substituting putative outer-loop residues adjacent to TM10 for the corresponding residues in GerVB, indicating that a degree of structural coordination between these regions is important to receptor function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.