ArticleJournal of bacteriology2009
A unique GTP-dependent sporulation sensor histidine kinase in Bacillus anthracis.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- A Dual Role for theFrontiers in microbiology · 2018Article
- The Pseudomonas aeruginosa AlgZR two-component system coordinates multiple phenotypes.Frontiers in cellular and infection microbiology · 2014Review
- Assembly of the BclB glycoprotein into the exosporium and evidence for its role in the formation of the exosporium 'cap' structure in Bacillus anthracis.Molecular microbiology · 2012Article
- From transcriptional landscapes to the identification of biomarkers for robustness.Microbial cell factories · 2011Review
- Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum.Molecular microbiology · 2011Article
- Autoregulatory characteristics of a Bacillus anthracis serine/threonine kinase.Journal of bacteriology · 2011Article
- Structural insights into inhibition of Bacillus anthracis sporulation by a novel class of non-heme globin sensor domains.The Journal of biological chemistry · 2011Article
- Novel two-component systems implied in antibiotic production in Streptomyces coelicolor.PloS one · 2011Article
- Structural and enzymatic insights into the ATP binding and autophosphorylation mechanism of a sensor histidine kinase.The Journal of biological chemistry · 2010Article
- Understanding the evolutionary relationships and major traits of Bacillus through comparative genomics.BMC genomics · 2010Article
- Regulatory interactions of a virulence-associated serine/threonine phosphatase-kinase pair in Bacillus anthracis.Journal of bacteriology · 2010Article
- A unique sensor histidine kinase.Journal of bacteriology · 2009Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
The Bacillus anthracis BA2291 gene codes for a sensor histidine kinase involved in the induction of sporulation. Genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay. Evidence suggests that the sensor domains inhibit BA2291 by titrating its activating signal ligand. Studies with purified BA2291 revealed that this kinase is uniquely specific for GTP in the forward reaction and GDP in the reverse reaction. The G1 motif of BA2291 is highly modified from ATP-specific histidine kinases, and modeling this motif in the structure of the kinase catalytic domain suggested how guanine binds to the region. A mutation in the putative coiled-coil linker between the sensor domain and the catalytic domains was found to decrease the rate of the forward autophosphorylation reaction and not affect the reverse reaction from phosphorylated Spo0F. The results suggest that the activating ligand for BA2291 is a critical signal for sporulation and in a limited concentration in the cell. Decreasing the response to it either by slowing the forward reaction through mutation or by titration of the ligand by expressing the plasmid-encoded sensor domains switches BA2291 from an inducer to an inhibitor of the phosphorelay and sporulation.
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