ArticleMethods (San Diego, Calif.)2008
Live cell imaging of phosphoinositides with expressed inositide binding protein domains.
Article in Methods (San Diego, Calif.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Expression of the GFP-mammalian pleckstrin homology (PH) domain of the phospholipase C δ1 in Saccharomyces cerevisiae BY4741.Molecular biology reports · 2022Article
- The Many Faces of Lipids in Genome Stability (and How to Unmask Them).International journal of molecular sciences · 2021Review
- A dual role for cell plate-associated PI4Kβ in endocytosis and phragmoplast dynamics during plant somatic cytokinesis.The EMBO journal · 2019Article
- NEDD4-induced degradative ubiquitination of phosphatidylinositol 4-phosphate 5-kinase α and its implication in breast cancer cell proliferation.Journal of cellular and molecular medicine · 2018Article
- The ARF guanine nucleotide exchange factor GBF1 is targeted to Golgi membranes through a PIP-binding domain.Journal of cell science · 2018Article
- Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes.Journal of visualized experiments : JoVE · 2016Article
- PtdIns4KIIα generates endosomal PtdIns(4)P and is required for receptor sorting at early endosomes.Molecular biology of the cell · 2016Article
- BRET-monitoring of the dynamic changes of inositol lipid pools in living cells reveals a PKC-dependent PtdIns4P increase upon EGF and M3 receptor activation.Biochimica et biophysica acta · 2016Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- A novel phosphatidylinositol 4,5-bisphosphate binding domain mediates plasma membrane localization of ExoU and other patatin-like phospholipases.The Journal of biological chemistry · 2015Article
- A method to control phosphoinositides and to analyze PTEN function in living cells using voltage sensitive phosphatases.Frontiers in pharmacology · 2015Article
- Sphingolipid homeostasis in the endoplasmic reticulum and beyond.Cold Spring Harbor perspectives in biology · 2013Review
- Actin-based confinement of calcium responses during Shigella invasion.Nature communications · 2013Article
- PI3K signalling in B- and T-lymphocytes: new developments and therapeutic advances.The Biochemical journal · 2012Review
- Palmitoylation targets AKAP79 protein to lipid rafts and promotes its regulation of calcium-sensitive adenylyl cyclase type 8.The Journal of biological chemistry · 2011Article
- Signaling at the Golgi.Cold Spring Harbor perspectives in biology · 2011Review
- Phosphoinositide 3-kinase regulates plasma membrane targeting of the Ras-specific exchange factor RasGRP1.The Journal of biological chemistry · 2011Article
- Effect of protein S-glutathionylation on Ca2+ homeostasis in cultured aortic endothelial cells.American journal of physiology. Heart and circulatory physiology · 2011Article
- A plasmid-based multigene expression system for mammalian cells.Nature communications · 2010Article
- Challenges in studying phospholipid signaling.Nature chemical biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Inositol lipids and calcium signaling has been inseparable twins during the 1980s when the molecular details of phospholipase C-mediated generation of inositol 1,4,5-trisphosphate (InsP3) and its Ca2+ mobilizing action were discovered. Since then, both the Ca2+ and inositol lipid signaling fields have hugely expanded and the tools allowing dissection of the finest details of their molecular organization also followed closely. Although phosphoinositides regulate many cell functions unrelated to Ca2+ signaling there are still many open questions even in the Ca2+ field that would benefit from single cell monitoring of PtdIns(4,5)P2 or InsP3 changes during agonist stimulation. This chapter is designed to provide practical guidance as well as some theoretical background on measurements of phosphoinositides in live cells using protein domain-GFP chimeras that could be also useful for people working on calcium signaling.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.