ArticleInternational journal for parasitology2009
Molecular cloning and DNA binding characterization of DAF-16 orthologs from Ancylostoma hookworms.
Article in International journal for parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- Three isoforms ofmicroPublication biology · 2025Article
- Altered larval activation response associated with multidrug resistance in the canine hookwormParasitology · 2024Article
- Article
- Plant-parasitic nematodes: towards understanding molecular players in stress responses.Annals of botany · 2017Review
- Signaling in Parasitic Nematodes: Physicochemical Communication Between Host and Parasite and Endogenous Molecular Transduction Pathways Governing Worm Development and Survival.Current clinical microbiology reports · 2016Article
- Expanding the view on the evolution of the nematode dauer signalling pathways: refinement through gene gain and pathway co-option.BMC genomics · 2016Article
- Molecular characterization of the Haemonchus contortus phosphoinositide-dependent protein kinase-1 gene (Hc-pdk-1).Parasites & vectors · 2016Article
- Two potential hookworm DAF-16 target genes, SNR-3 and LPP-1: gene structure, expression profile, and implications of a cis-regulatory element in the regulation of gene expression.Parasites & vectors · 2015Article
- Exploring the role of two interacting phosphoinositide 3-kinases of Haemonchus contortus.Parasites & vectors · 2014Article
- Hc-daf-2 encodes an insulin-like receptor kinase in the barber's pole worm, Haemonchus contortus, and restores partial dauer regulation.International journal for parasitology · 2014Article
- The dauer hypothesis and the evolution of parasitism: 20 years on and still going strong.International journal for parasitology · 2014Review
- Strongyloides stercoralis daf-2 encodes a divergent ortholog of Caenorhabditis elegans DAF-2.International journal for parasitology · 2013Article
- Strongyloides stercoralis age-1: a potential regulator of infective larval development in a parasitic nematode.PloS one · 2012Article
- RNA and protein synthesis is required for Ancylostoma caninum larval activation.Veterinary parasitology · 2011Article
- Characterisation of hookworm heat shock factor binding protein (HSB-1) during heat shock and larval activation.International journal for parasitology · 2011Article
- Hsp12.6 expression is inducible by host immunity in adult worms of the parasitic nematode Nippostrongylus brasiliensis.PloS one · 2011Article
- Transgenic C. elegans dauer larvae expressing hookworm phospho null DAF-16/FoxO exit dauer.PloS one · 2011Article
- Article
- Structural and functional characterisation of the fork head transcription factor-encoding gene, Hc-daf-16, from the parasitic nematode Haemonchus contortus (Strongylida).International journal for parasitology · 2010Article
- Interaction of hookworm 14-3-3 with the forkhead transcription factor DAF-16 requires intact Akt phosphorylation sites.Parasites & vectors · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Infective hookworm L3s encounter a host-specific signal during infection that re-initiates a suspended developmental pathway, resulting in development to the adult stage. This resumption of development in the host is analogous to recovery of developmentally arrested Caenorhabditis elegans dauer larvae in response to favorable environmental signals. Dauer recovery in C. elegans dauers and hookworm L3s is mediated by insulin-like signaling (ILS). A key output of ILS in C. elegans is the forkhead transcription factor DAF-16, which controls the expression of genes required for maintenance of the dauer stage. The similarity between recovery pathways of L3s and dauers suggests that DAF-16 functions similarly in hookworm L3 activation. To test this, orthologs of Ce-DAF-16 were isolated from the hookworms Ancylostoma caninum and Ancylostoma ceylanicum. The protein sequences of hookworm DAF-16 DNA binding domains were identical, and shared 94% identity with the b and c isoforms of Ce-DAF-16. Ac-DAF-16 expressed in HEK293 kidney cells bound strongly to the conserved DAF family binding element (DBE), but not to a random DNA sequence. Ac-DAF-16 was able to drive transcription of a reporter gene located downstream of six copies of the DBE in NIH3T3 cells under starved conditions. Addition of serum caused a decrease in reporter gene expression, indicating that DAF-16 is negatively regulated by growth factor stimulation. These data confirm the presence of DAF-16 orthologs in hookworms, and demonstrate that Ac-DAF-16 binds to and drives transcription from a conserved DAF-16 family DNA binding element.
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