ArticleBiomaterials2009
Layered PLG scaffolds for in vivo plasmid delivery.
Article in Biomaterials, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Gene-Activated Materials in Regenerative Dentistry: Narrative Review of Technology and Study Results.International journal of molecular sciences · 2023Review
- Extrahepatic transplantation of 3D cultured stem cell-derived islet organoids on microporous scaffolds.Biomaterials science · 2023Article
- Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine.Annals of biomedical engineering · 2014Review
- Enhancing human islet transplantation by localized release of trophic factors from PLG scaffolds.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2014Article
- Effect of pore structure of macroporous poly(lactide-co-glycolide) scaffolds on the in vivo enrichment of dendritic cells.ACS applied materials & interfaces · 2014Article
- Modulation of leukocyte infiltration and phenotype in microporous tissue engineering scaffolds via vector induced IL-10 expression.Biomaterials · 2014Article
- Biomaterial Scaffolds for Controlled, Localized Gene Delivery of Regenerative Factors.Advances in wound care · 2013Review
- Porous scaffolds support extrahepatic human islet transplantation, engraftment, and function in mice.Cell transplantation · 2013Article
- Extrahepatic islet transplantation with microporous polymer scaffolds in syngeneic mouse and allogeneic porcine models.Biomaterials · 2011Article
- Advancing islet transplantation: from engraftment to the immune response.Diabetologia · 2011Review
- Vascular endothelial growth factor and fibroblast growth factor 2 delivery from spinal cord bridges to enhance angiogenesis following injury.Journal of biomedical materials research. Part A · 2011Article
- Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions.Biomaterials · 2011Article
- Hydrogels to modulate lentivirus delivery in vivo from microporous tissue engineering scaffolds.Drug delivery and translational research · 2011Article
- Gene delivery by surface immobilization of plasmid to tissue-engineering scaffolds.Gene therapy · 2010Article
- Patterned transgene expression in multiple-channel bridges after spinal cord injury.Acta biomaterialia · 2010Article
- Lentivirus delivery by adsorption to tissue engineering scaffolds.Journal of biomedical materials research. Part A · 2010Article
- The contribution of plasmid design and release to in vivo gene expression following delivery from cationic polymer modified scaffolds.Biomaterials · 2010Article
- Sustained transgene expression via citric acid-based polyester elastomers.Biomaterials · 2009Article
- Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury.Biomaterials · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Gene delivery from tissue engineering scaffolds can induce localized expression of tissue inductive factors to direct the function of progenitor cells, either endogenous or transplanted. In this report, we developed a layering approach for fabricating scaffolds with encapsulated plasmid, and investigated in vivo gene transfer following implantation into intraperitoneal fat, a widely used site for cell transplantation. Porous poly(lactide-co-glycolide) (PLG) scaffolds were fabricated using a gas foaming method, in which a non-porous layer containing plasmid was inserted between two porous polymer layers. The layered scaffold design decouples the scaffold structural requirements from its function as a drug delivery vehicle, and significantly increased the plasmid incorporation efficiency relative to scaffolds formed without layers. For multiple plasmid doses (200, 400, and 800mug), transgene expression levels peaked during the first few days and then declined over a period of 1-2 weeks. Transfected cells were observed both in the surrounding adipose tissue and within the scaffold interior. Macrophages were identified as an abundantly transfected cell type. Scaffolds delivering plasmid encoding fibroblast growth factor-2 (FGF-2) stimulated a 40% increase in the total vascular volume fraction relative to controls at 2 weeks. Scaffold-based gene delivery systems capable of localized transgene expression provide a platform for inductive and cell transplantation approaches in regenerative medicine.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.