ArticleJournal of medical microbiology2008
Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping.
Article in Journal of medical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed.
- Susceptibility of Clostridium difficile isolates from a Phase 2 clinical trial of cadazolid and vancomycin in C. difficile infection.The Journal of antimicrobial chemotherapy · 2016Trial
- Genomic and phenotypic insight intoEmerging microbes & infections · 2026Article
- Clostridioides difficile as a Zoonotic and Foodborne Pathogen: Reviewing the Global Evidence and South African Data.MicrobiologyOpen · 2026Review
- Non-targeted metabolomics reveals metabolic signatures associated withFrontiers in microbiology · 2026Article
- Automated web-based typing of Clostridioides difficile ribotypes via MALDI-TOF MS.BMC bioinformatics · 2025Article
- The phi027 bacteriophage influences physiology and virulence of the lysogenic strain of Clostridioides difficile.Scientific reports · 2025Article
- Analysis of high-molecular-weight proteins using MALDI-TOF MS and machine learning for the differentiation of clinically relevant Clostridioides difficile ribotypes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Pilot study ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- In-depth characterization of accessory gene regulator loci and associated virulence factors inCurrent research in microbial sciences · 2025Article
- Genomic epidemiology ofEmerging microbes & infections · 2024Article
- Usefulness of Capillary Gel Electrophoresis-Based PCR for Detection ofGels (Basel, Switzerland) · 2024Article
- Gut microbiome changes in mouse, Mongolian gerbil, and hamster models followingFrontiers in microbiology · 2024Article
- Ribotypes and New Virulent Strains Across Europe.Advances in experimental medicine and biology · 2024Review
- Discrimination between hypervirulent and non-hypervirulent ribotypes of Clostridioides difficile by MALDI-TOF mass spectrometry and machine learning.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Article
- Characterization of Food ChainMicroorganisms · 2023Article
- Membrane Vesicles of ToxigenicAntioxidants (Basel, Switzerland) · 2023Article
- Novel ribotype/sequence type associations and diverse CRISPR-Cas systems in environmental Clostridioides difficile strains from northern Iraq.FEMS microbiology letters · 2023Article
- Article
- Prevalence of diagnostically-discrepantFrontiers in medicine · 2023Article
45 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a Clostridium difficile PCR ribotyping method based on capillary gel electrophoresis and have compared it with conventional PCR ribotyping. A total of 146 C. difficile isolates were studied: five isolates were reference strains (PCR ribotypes 001, 014, 017, 027 and 053); 141 were clinical isolates comprising 39 Austrian PCR ribotypes collected in the period 2006-2007 at 25 Austrian healthcare facilities. Capillary gel electrophoresis yielded up to 11 fragments per isolate and 47 ribotype patterns. All but one of the five PCR ribotypes of reference strains were clearly reflected in the chromatograms of capillary-based typing. Capillary gel electrophoresis divided 24 isolates belonging to PCR ribotype type 014 into seven subgroups, whereas subtyping the same isolates using multiple-locus variable-number tandem-repeat analysis yielded three unrelated subgroups, without obvious correlation to sr subgroups. Using a web-based software program (http://webribo.ages.at), we were able to correctly identify these 014 isolates by simply allocating the seven subgroup patterns to one ribotype, i.e. to PCR ribotype 014. We consider capillary gel electrophoresis-based PCR ribotyping to be a way of overcoming the problems associated with inter-laboratory comparisons of typing results, while at the same time substantially diminishing the hands-on time for PCR ribotyping.
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