ArticleAmerican journal of respiratory cell and molecular biology2009
Expression and regulation of epithelial Na+ channels by nucleotides in pleural mesothelial cells.
Article in American journal of respiratory cell and molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Competitive cleavage of SARS-CoV-2 spike protein and epithelial sodium channel by plasmin as a potential mechanism for COVID-19 infection.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy.Annals of translational medicine · 2022Article
- Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis.International journal of molecular sciences · 2021Article
- Transepithelial Fluid and Salt Re-Absorption Regulated by cGK2 Signals.International journal of molecular sciences · 2018Review
- Novel mechanisms for crotonaldehyde-induced lung edema.Oncotarget · 2017Article
- Formaldehyde impairs transepithelial sodium transport.Scientific reports · 2016Article
- Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro.Pflugers Archiv : European journal of physiology · 2015Article
- Regulation of epithelial sodium channels in urokinase plasminogen activator deficiency.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Tight junction physiology of pleural mesothelium.Frontiers in physiology · 2014Review
- δ ENaC: a novel divergent amiloride-inhibitable sodium channel.American journal of physiology. Lung cellular and molecular physiology · 2012Review
- Permeability of the arachnoid and pia mater. The role of ion channels in the leptomeningeal physiology.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2012Article
- Ketamine inhibits lung fluid clearance through reducing alveolar sodium transport.Journal of biomedicine & biotechnology · 2011Article
- Dexamethasone acutely accelerates pleural fluid absorption in mice hydrothoraces.The journal of physiological sciences : JPS · 2010Article
- 8-pCPT-cGMP stimulates alphabetagamma-ENaC activity in oocytes as an external ligand requiring specific nucleotide moieties.American journal of physiology. Renal physiology · 2010Article
- The delta-subunit of the epithelial sodium channel (ENaC) enhances channel activity and alters proteolytic ENaC activation.The Journal of biological chemistry · 2009Article
- Dexamethasone decreases the transmesothelial electrical resistance of the parietal and visceral pleura.The journal of physiological sciences : JPS · 2009Article
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
Pleural effusions are commonly clinical disorders, resulting from the imbalance between pleural fluid turnover and reabsorption. The mechanisms underlying pleural fluid clearance across the mesothelium remain to be elucidated. We hypothesized that epithelial Na(+) channel (ENaC) is expressed and forms the molecular basis of the amiloride-sensitive resistance in human mesothelial cells. Our RT-PCR results showed that three ENaC subunits, namely, alpha, beta, gamma, and two delta ENaC subunits, are expressed in human primary pleural mesothelial cells, a human mesothelioma cell line (M9K), and mouse pleural tissue. In addition, Western blotting and immunofluorescence microscopy studies revealed that alpha, beta, gamma, and delta ENaC subunits are expressed in primary human mesothelial cells and M9K cells at the protein level. An amiloride-inhibitable short-circuit current was detected in M9K monolayers and mouse pleural tissues when mounted in Ussing chambers. Whole-cell patch clamp recordings showed an ENaC-like channel with an amiloride concentration producing 50% inhibition of 12 microM in M9K cells. This cation channel has a high affinity for extracellular Na+ ions (K(m): 53 mM). The ion selectivity of this channel to cations follows the same order as ENaC: Li+ > Na+ > K+. The unitary Li(+) conductance was 15 pS in on-cell patches. Four ENaC subunits form a functional Na+ channel when coinjected into Xenopus oocytes. Furthermore, we found that both forskolin and cGMP increased the short-circuit currents in mouse pleural tissues. Taken together, our data demonstrate that the ENaC channels are biochemically and functionally expressed in human pleural mesothelial cells, and can be up-regulated by cyclic AMP and cyclic GMP.
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