ArticleClinical and vaccine immunology : CVI2008
Multiplex immunoassay for serological diagnosis of Mycobacterium bovis infection in cattle.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Accuracy of Tests for Diagnosis of Animal Tuberculosis: Moving Away from the Golden Calf (and towards Bayesian Models).Transboundary and emerging diseases · 2023Pooled it
- Recombinant proteins expressed in Mycolicibacterium smegmatis enhance antibody-based detection of bovine tuberculosis.Veterinary research · 2026Article
- B Cell and Antibody Responses in Bovine Tuberculosis.Antibodies (Basel, Switzerland) · 2024Review
- Diagnostic accuracy of the Enferplex Bovine TB antibody test using individual milk samples from cattle.PloS one · 2024Article
- Phylogenetic analysis of prospective M. bovis antigens with the aim of developing candidate vaccines for bovine tuberculosis.Journal, genetic engineering & biotechnology · 2023Article
- Safety and Protective Efficacy of a Candidate Vector-Based Vaccine for Bovine Tuberculosis.Vaccines · 2023Article
- Article
- Serological test performance for bovine tuberculosis in cattle from herds with evidence of on-going infection in Northern Ireland.PloS one · 2021Article
- Prevalence and Demographic Risk Factors ofFrontiers in veterinary science · 2021Article
- Application of Volatilome Analysis to the Diagnosis of Mycobacteria Infection in Livestock.Frontiers in veterinary science · 2021Review
- Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations.BMC veterinary research · 2019Article
- Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test-negative dairy cattle in Kuwait.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results.BMC veterinary research · 2017Article
- Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test.Clinical and vaccine immunology : CVI · 2017Article
- Control of bovine tuberculosis in a farmed red deer herd in England.The Veterinary record · 2017Article
- Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas.Malaria journal · 2015Article
- Effects of Serial Skin Testing with Purified Protein Derivative on the Level and Quality of Antibodies to Complex and Defined Antigens in Mycobacterium bovis-Infected Cattle.Clinical and vaccine immunology : CVI · 2015Article
- Epidemiology, diagnostics, and management of tuberculosis in domestic cattle and deer in New Zealand in the face of a wildlife reservoir.New Zealand veterinary journal · 2015Review
- Evaluation of ethanol vortex ELISA for detection of bovine tuberculosis in cattle and deer.BMC veterinary research · 2014Article
- Article
Corrections and comments
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Authors and funding
20 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efforts to develop a better diagnostic assay for bovine tuberculosis have shown that the sensitivity and specificity of an assay can be improved by the use of two or more antigens. As reported here, we developed a multiplex chemiluminescent immunoassay that can simultaneously detect antibody activity to 25 antigens in a single well in a 96-well plate array format. The chemiluminescent signal is captured with a digital imaging system and analyzed with a macro program that tracks each serum for its pattern of antibody activity for Mycobacterium bovis antigens. The comparison of sera from 522 infected and 1,489 uninfected animals showed that a sensitivity of 93.1% and a specificity of 98.4% can be achieved with a combination of antigens. The assay system is rapid and can be automated for use in a centralized laboratory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.