ArticleClinical and molecular allergy : CMA2008
Differential response of human basophil activation markers: a multi-parameter flow cytometry approach.
Article in Clinical and molecular allergy : CMA, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 85 citations in OpenAlex.
- Comprehensive immune profiling of human peripheral blood mononuclear cells using two complementary spectral flow cytometry panels encompassing 60 unique markers.Frontiers in immunology · 2026Article
- A hand-operated microfluidic sample preparation-to-analysis workflow for simplifying the basophil activation test.Lab on a chip · 2025Article
- Role of the basophil activation test in the diagnosis and management of immediate drug reaction to injectable growth hormone preparation: a case report.Immunologic research · 2025Article
- Diagnostic Use of CCR3, CD63, CD203c and FcεRIα on Blood Leukocytes of Allergic Asthma and Combined Allergic Rhinitis and Asthma Syndrome.Journal of cellular and molecular medicine · 2025Article
- Basophils in pruritic skin diseases.Frontiers in immunology · 2023Review
- Towards standardizing basophil identification by flow cytometry.Frontiers in allergy · 2023Article
- E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages.Biomolecules · 2022Article
- A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity.BMC medicine · 2022Article
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- Diverse innate stimuli activate basophils through pathways involving Syk and IκB kinases.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Behind the scenes with basophils: an emerging therapeutic target.Immunotherapy advances · 2021Review
- Road map for the clinical application of the basophil activation test in food allergy.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2017Review
- Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis.The Journal of allergy and clinical immunology · 2017Article
- Basophil activation test in oral desensitization to cow's milk allergy.United European gastroenterology journal · 2016Article
- Basophil Activation Test for Chronic Urticaria.Annals of laboratory medicine · 2016Article
- A novel approach to predict cetuximab-induced hypersensitivity reaction: detection of drug-specific IgE on basophils.Cancer medicine · 2016Article
- The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test.Clinical and translational allergy · 2016Article
- Basophil activation test: food challenge in a test tube or specialist research tool?Clinical and translational allergy · 2016Review
- Commentary: The Expression of CD123 Can Decrease with Basophil Activation: Implications for the Gating Strategy of the Basophil Activation Test.Frontiers in immunology · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBasophils are circulating cells involved in hypersensitivity reactions and allergy but many aspects of their activation, including the sensitivity to external triggering factors and the molecular aspects of cell responses, are still to be focused. In this context, polychromatic flow cytometry (PFC) is a proper tool to investigate basophil function, as it allows to distinguish the expression of several membrane markers upon activation in multiple experimental conditions.
methodsCell suspensions were prepared from leukocyte buffy coat of K2-EDTA anticoagulated blood specimens; about 1500-2500 cellular events for each tested sample, gated in the lymphocyte CD45dim area and then electronically purified as HLADRnon expressing/CD123bright, were identified as basophilic cells. Basophil activation with fMLP, anti-IgE and calcium ionophore A23187 was evaluated by studying up-regulation of the indicated membrane markers with a two-laser six-color PFC protocol.
resultsFollowing stimulation, CD63, CD13, CD45 and the ectoenzyme CD203c up-regulated their membrane expression, while CD69 did not; CD63 expression occurred immediately (within 60 sec) but only in a minority of basophils, even at optimal agonist doses (in 33% and 14% of basophils, following fMLP and anti-IgE stimulation respectively). CD203c up-regulation occurred in the whole basophil population, even in CD63non expressing cells. Dose-dependence curves revealed CD203c as a more sensitive marker than CD63, in response to fMLP but not in response to anti-IgE and to calcium ionophore.
conclusionUse of polychromatic flow cytometry allowed efficient basophil electronic purification and identification of different behaviors of the major activation markers. The simultaneous use of two markers of activation and careful choice of activator are essential steps for reliable assessment of human basophil functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.