Evidence map›Paper›PMID 18923920›Full record

ArticleMolecular biology reports2009

Molecular cloning and characterization of a 2C-methyl-D: -erythritol 2,4-cyclodiphosphate synthase gene from Cephalotaxus harringtonia.

Junfeng Chen, Ying Xiao, Peng Di, Xiaojing Yu, Wansheng Chen, Lei Zhang

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Article in Molecular biology reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Methyl Jasmonate Activates theMolecules (Basel, Switzerland) · 2022
    Article
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Junfeng ChenDepartment of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China.
Ying Xiao
Peng Di
Xiaojing Yu
Wansheng Chen
Lei Zhang

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The full-length MECPS cDNA sequence (designated as Chmecps, GenBank Accession No.: DQ415658) was isolated by rapid amplification of cDNA ends (RACE) for the first time from Cephalotaxus harringtonia. The full-length cDNA of Chmecps was 1,146 bp containing a 753 bp open reading frame (ORF) encoding a polypeptide of 250 amino acids with a calculated mass of 26.67 kDa and an isoelectric point of 9.35. Comparative and bioinformatics analyses revealed that ChMECPS showed extensive homology with MECPSs from other plant species. Phylogenetic analysis indicated ChMECPS was more ancient than other plant MECPSs. Southern hybridization analysis of the genomic DNA showed that Chmecps was a single copy gene. Tissue expression pattern analysis revealed that ChMECPS expressed strongly in root and leaf, weakly in stem.

Indexed as

Genes, PlantAmino Acid SequenceBase SequenceBlotting, SouthernCephalotaxusCloning, MolecularComputational BiologyDNA, ComplementaryEvolution, MolecularGene Expression ProfilingGene Expression Regulation, EnzymologicGene Expression Regulation, PlantMolecular Sequence DataPhosphorus-Oxygen LyasesPhylogenyProtein Structure, SecondaryDNA, ComplementaryPhosphorus-Oxygen Lyases

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.