ArticleThe EMBO journal2008
Regulation of ARE transcript 3' end processing by the yeast Cth2 mRNA decay factor.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Nuclear Microautophagy Drives Vacuolar Targeting of Yeast Iron-Regulated Proteins During Lipid and Iron Limitation.MicrobiologyOpen · 2026Article
- Regulatory and pathogenic mechanisms in response to iron deficiency and excess in fungi.Microbial biotechnology · 2023Review
- EGOC inhibits TOROID polymerization by structurally activating TORC1.Nature structural & molecular biology · 2023Article
- Iron Regulatory Mechanisms inFrontiers in microbiology · 2020Review
- Dissecting mRNA decay and translation inhibition during iron deficiency.Current genetics · 2019Review
- Yeast Cth2 protein represses the translation of ARE-containing mRNAs in response to iron deficiency.PLoS genetics · 2018Article
- Yeast Dun1 Kinase Regulates Ribonucleotide Reductase Small Subunit Localization in Response to Iron Deficiency.The Journal of biological chemistry · 2016Article
- Cellular sensing and transport of metal ions: implications in micronutrient homeostasis.The Journal of nutritional biochemistry · 2015Review
- Yeast Dun1 kinase regulates ribonucleotide reductase inhibitor Sml1 in response to iron deficiency.Molecular and cellular biology · 2014Article
- Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice.Plant molecular biology · 2014Article
- Post-transcriptional regulation of iron homeostasis in Saccharomyces cerevisiae.International journal of molecular sciences · 2013Review
- Negative feedback regulation of the yeast CTH1 and CTH2 mRNA binding proteins is required for adaptation to iron deficiency and iron supplementation.Molecular and cellular biology · 2013Article
- Multiple functions of tristetraprolin/TIS11 RNA-binding proteins in the regulation of mRNA biogenesis and degradation.Cellular and molecular life sciences : CMLS · 2013Review
- Article
- Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.Molecular and cellular biology · 2012Article
- Metabolic remodeling in iron-deficient fungi.Biochimica et biophysica acta · 2012Review
- Article
- A novel function of Tis11b/BRF1 as a regulator of Dll4 mRNA 3'-end processing.Molecular biology of the cell · 2011Article
- Understanding the transcriptome through RNA structure.Nature reviews. Genetics · 2011Review
- An RNA-protein complex links enhanced nuclear 3' processing with cytoplasmic mRNA stabilization.The EMBO journal · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of mRNA decay is an important step modulating gene expression. The stability of numerous eukaryotic mRNAs is controlled by adenosine/uridine-rich elements (AREs) located in their 3'UTR. In Saccharomyces cerevisiae, the Cth2 protein stimulates the decay of target ARE mRNAs on iron starvation. Cth2, and its mammalian homologue tristetraprolin, contains a characteristic tandem CCCH zinc-finger essential for ARE binding and mRNA decay. We have performed a structure-function analysis of Cth2 to understand the mechanism(s) by which it destabilizes mRNAs. This indicated that a conserved N-terminal region of Cth2 is essential for its decay function but dispensable for RNA binding. Unexpectedly, Cth2 mutants lacking this domain blocked the normal 3' end processing of ARE mRNAs leading to the formation of extended transcripts. These can also be detected in mutant of the polyadenylation machinery. Consistently, Cth2 localization in the nucleus suggests that it may interfere with poly(A) site selection. Our analysis reveal that ARE-binding protein may affect mRNA 3' end processing and that this contributes to mRNA destabilization.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.