ArticleThe EMBO journal2008
The IQGAP Iqg1 is a regulatory target of CDK for cytokinesis in Candida albicans.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Cytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagataella phaffii.Microbial cell factories · 2024Article
- Unraveling the mechanisms and evolution of a two-domain module in IQGAP proteins for controlling eukaryotic cytokinesis.Cell reports · 2023Article
- scGET: Predicting Cell Fate Transition During Early Embryonic Development by Single-cell Graph Entropy.Genomics, proteomics & bioinformatics · 2021Article
- Tyrosine phosphorylation of the scaffold protein IQGAP1 in the MET pathway alters function.The Journal of biological chemistry · 2020Article
- Transcriptome Analyses ofG3 (Bethesda, Md.) · 2020Article
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
- The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae.Cellular and molecular life sciences : CMLS · 2016Review
- Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.Nature communications · 2016Article
- Dephosphorylation of Iqg1 by Cdc14 regulates cytokinesis in budding yeast.Molecular biology of the cell · 2015Article
- The biology of IQGAP proteins: beyond the cytoskeleton.EMBO reports · 2015Review
- IQGAP1 is associated with nuclear envelope reformation and completion of abscission.Cell cycle (Georgetown, Tex.) · 2015Article
- Novel mechanism coupling cyclic AMP-protein kinase A signaling and golgi trafficking via Gyp1 phosphorylation in polarized growth.Eukaryotic cell · 2014Article
- Cdk1-dependent phosphorylation of Iqg1 governs actomyosin ring assembly prior to cytokinesis.Journal of cell science · 2014Article
- Cell cycle regulation of Rho signaling pathways.Cell cycle (Georgetown, Tex.) · 2012Review
- IQGAP Family Members in Yeast, Dictyostelium, and Mammalian Cells.International journal of cell biology · 2012Article
- Cdc28 provides a molecular link between Hsp90, morphogenesis, and cell cycle progression in Candida albicans.Molecular biology of the cell · 2012Article
- Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- IQ-motif selectivity in human IQGAP2 and IQGAP3: binding of calmodulin and myosin essential light chain.Bioscience reports · 2011Article
- On the cutting edge: post-translational modifications in cytokinesis.Trends in cell biology · 2011Review
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclin-dependent kinases (CDKs) drive and coordinate multiple cell-cycle events, including construction and contraction of the actomyosin ring during cytokinesis. However, it remains unclear whether CDKs regulate cytokinesis by directly targeting components of the ring. In a search for proteins containing consensus CDK phosphorylation sites in Candida albicans, we found that the IQGAP Iqg1 contains two dense clusters of 19 such sites flanking the actin-interacting CH domain. Here, we show that Iqg1 is indeed a phosphoprotein that undergoes cell-cycle-dependent phosphorylation and can be phosphorylated by purified Clb-Cdc28 kinases in vitro. Mass spectrometry identified several phosphoserine and phosphothreonine residues among these CDK sites. Mutating 15 of the CDK phosphorylation sites with alanine markedly reduced Iqg1 phosphorylation in vivo. The 15A mutation greatly stabilized Iqg1, caused both premature assembly and delayed disassembly of the actomyosin ring, blocked Iqg1 interaction with the actin-nucleating proteins Bni1 and Bnr1, and resulted in defects in cytokinesis. Our data therefore strongly support the idea that the Cdc28 CDK regulates cytokinesis partly by directly phosphorylating the actomyosin ring component Iqg1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.