ArticleJournal of clinical microbiology2008
Rapid multiplex PCR and real-time TaqMan PCR assays for detection of Salmonella enterica and the highly virulent serovars Choleraesuis and Paratyphi C.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
- Major Foodborne Bacterial Pathogens in Poultry: Implications for Human Health and the Poultry Industry and Probiotic Mitigation Strategies.Microorganisms · 2025Review
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- Identification of Salmonella enterica biovars Gallinarum and Pullorum and their antibiotic resistance pattern in integrated crop-livestock farms and poultry meats.Access microbiology · 2024Article
- Novel Heptaplex PCR-Based Diagnostics for Enteric Fever Caused by TyphoidalJournal of microbiology and biotechnology · 2023Article
- Human Salmonellosis: A Continuous Global Threat in the Farm-to-Fork Food Safety Continuum.Foods (Basel, Switzerland) · 2023Review
- Assessment of the prevalence, serotype, and antibiotic resistance pattern ofFrontiers in microbiology · 2023Article
- Frequency of Salmonella serotypes among children in Iran: antimicrobial susceptibility, biofilm formation, and virulence genes.BMC pediatrics · 2022Article
- Classification ofMicroorganisms · 2021Article
- Genome-based Salmonella serotyping as the new gold standard.Scientific reports · 2020Article
- Designing a biochip following multiplex polymerase chain reaction for the detection of Salmonella serovars Typhimurium, Enteritidis, Infantis, Hadar, and Virchow in poultry products.Journal of food and drug analysis · 2018Article
- Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens.Transboundary and emerging diseases · 2017Article
- Ultra-fast and sensitive detection of non-typhoidal Salmonella using microwave-accelerated metal-enhanced fluorescence ("MAMEF").PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica is a human pathogen with over 2,500 serovars characterized. S. enterica serovars Choleraesuis and Paratyphi C are two globally distributed serovars. We have developed a rapid molecular-typing method to detect serovars Choleraesuis and Paratyphi C in food samples by using a comparative-genomics approach to identify regions unique to each serovar from the sequenced genomes. A Salmonella-specific primer pair based on oriC was designed as an internal control to establish accuracy, sensitivity, and reproducibility. Serovar-specific primer sets based on regions of difference between serovars Choleraesuis and Paratyphi C were designed for real-time PCR assays. Three primer sets were used to screen a collection of over 100 Salmonella strains, and both serovars Choleraesuis and Paratyphi C gave unique amplification patterns. To develop the technique for practical use, its sensitivity for detection of Salmonella spp. in a food matrix was determined by spiking experiments. The technique was also adapted for a real-time PCR rapid-detection assay for both serovars Choleraesuis and Paratyphi C that complements the current procedures for Salmonella sp. isolation and serotyping.
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Registered trials
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