ArticleJournal of clinical microbiology2008
Real-time PCR with an internal control for detection of all known human adenovirus serotypes.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Trial
- Microscopic Observation of SARS-Like Particles in RT-qPCR SARS-CoV-2 Positive Sewage Samples.Pathogens (Basel, Switzerland) · 2021Article
- Microfluidic-based virus detection methods for respiratory diseases.Emergent materials · 2021Review
- Validation of a Laboratory-Developed Triplex Molecular Assay for Simultaneous Detection of Gastrointestinal Adenovirus and Rotavirus in Stool Specimens.Pathogens (Basel, Switzerland) · 2020Article
- Deposition of respiratory virus pathogens on frequently touched surfaces at airports.BMC infectious diseases · 2018Article
- Identification of Soil Bacterial Isolates Suppressing DifferentFrontiers in plant science · 2018Article
- Severe Respiratory Viral Infections: New Evidence and Changing Paradigms.Infectious disease clinics of North America · 2017Review
- Aetiology and Outcomes of Suspected Infections of the Central Nervous System in Children in Mbarara, Uganda.Scientific reports · 2017Article
- Clinical characteristics and factors predicting respiratory failure in adenovirus pneumonia.Respirology (Carlton, Vic.) · 2016Article
- Throat and nasal swabs for molecular detection of respiratory viruses in acute pharyngitis.Virology journal · 2015Article
- Fulminant isolated adenovirus hepatitis 5 months after haplo-identical HSCT for AML.Clinical case reports · 2015Article
- Human respiratory syncytial virus in children with lower respiratory tract infections or influenza-like illness and its co-infection characteristics with viruses and atypical bacteria in Hangzhou, China.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015Article
- Outbreak of coinfection with human metapneumovirus and measles virus resulting in the death of a child at a hospital in China.American journal of infection control · 2015Article
- Outcomes of early administration of cidofovir in non-immunocompromised patients with severe adenovirus pneumonia.PloS one · 2015Article
- Evaluation of anatomically designed flocked rectal swabs for molecular detection of enteric pathogens in children admitted to hospital with severe gastroenteritis in Botswana.Journal of clinical microbiology · 2014Article
- Detection of pathogens in water: from phylochips to qPCR to pyrosequencing.Current opinion in biotechnology · 2012Review
- Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources.Journal of clinical microbiology · 2012Article
- Prior infections with seasonal influenza A/H1N1 virus reduced the illness severity and epidemic intensity of pandemic H1N1 influenza in healthy adults.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Development and utility of an internal threshold control (ITC) real-time PCR assay for exogenous DNA detection.PloS one · 2012Article
- Evaluation and verification of the Seeplex Diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in clinical stool specimens.Journal of clinical microbiology · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The "gold standard" for the diagnosis of adenovirus (AV) infection is virus culture, which is rather time-consuming. Especially for immunocompromised patients, in whom severe infections with AV have been described, rapid diagnosis is important. Therefore, an internally controlled AV real-time PCR assay detecting all known human AV serotypes was developed. Primers were chosen from the hexon region, which is the most conserved region, and in order to cover all known serotypes, degenerate primers were used. The internal control (IC) DNA contained the same primer binding sites as the AV DNA control but had a shuffled probe region compared to the conserved 24-nucleotide consensus AV hexon probe region (the target). The IC DNA was added to the clinical sample in order to monitor extraction and PCR efficiency. The sensitivity and the linearity of the AV PCR were determined. For testing the specificity of this PCR assay for human AVs, a selection of 51 AV prototype strains and 66 patient samples positive for other DNA viruses were tested. Moreover, a comparison of the AV PCR method described herein with culture and antigen (Ag) detection was performed with a selection of 151 clinical samples. All 51 AV serotypes were detected in the selection of AV prototype strains. Concordant results from culture or Ag detection and PCR were found for 139 (92.1%) of 151 samples. In 12 cases (7.9%), PCR was positive while the culture was negative. In conclusion, a sensitive, internally controlled nonnested AV real-time PCR assay which is able to detect all known AV serotypes with higher sensitivity than a culture or Ag detection method was developed.
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