ArticleThe Journal of biological chemistry2008
Requirements for protein phosphorylation and the kinase activity of polo-like kinase 1 (Plk1) for the kinetochore function of mitotic arrest deficiency protein 1 (Mad1).
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Phosphorylation of Mad1 at serine 18 by Mps1 is required for the full virulence of rice blast fungus, Magnaporthe oryzae.Molecular plant pathology · 2024Article
- ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.Nucleic acids research · 2019Article
- Direct interactions of mitotic arrest deficient 1 (MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling.The Journal of biological chemistry · 2018Article
- MAD1: Kinetochore Receptors and Catalytic Mechanisms.Frontiers in cell and developmental biology · 2018Review
- ATM-mediated Mad1 Serine 214 phosphorylation regulates Mad1 dimerization and the spindle assembly checkpoint.Carcinogenesis · 2014Article
- Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation.The EMBO journal · 2013Article
- Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Systematic phosphorylation analysis of human mitotic protein complexes.Science signaling · 2011Article
- Suppression of genomic instabilities caused by chromosome mis-segregation: a perspective from studying BubR1 and Sgo1.Journal of the Formosan Medical Association = Taiwan yi zhi · 2009Review
- The nuclear envelopathies and human diseases.Journal of biomedical science · 2009Review
- Viral transformation and aneuploidy.Environmental and molecular mutagenesis · 2009Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Mitotic arrest deficiency protein 1 (Mad1) is associated with microtubule-unattached kinetochores in mitotic cells and is a component of the spindle assembly checkpoint (SAC). Here, we have studied the phosphorylation of Mad1 and mapped using liquid chromatography-tandem mass spectrometry several phosphorylated amino acids in this protein. One phosphorylated residue, Thr680, was characterized to be important for the kinetochore localization of Mad1 and its SAC function. We also found that in mitotic cells Mad1 co-immunoprecipitated with Plk1. Depletion of cellular Plk1 using small interfering RNAs and inhibition of the kinase activity of Plk1 using a kinase-dead mutant or a small molecule inhibitor attenuated Mad1 phosphorylation and its association with kinetochores. Collectively, these findings indicate mechanistic roles contributed by protein phosphorylation and Plk1 to the SAC activity of Mad1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.