ArticleProceedings of the National Academy of Sciences of the United States of America2008
Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 78 citations in OpenAlex.
- Review
- Sulfolobales: Acidothermophilic archaea as models for biology and biotechnological applications.Engineering microbiology · 2026Review
- Coordination of chromosome segregation and cell division in the archaeon Sulfolobus acidocaldarius.Nature communications · 2025Article
- An archaeal nucleoid-associated protein binds an essential motif in DNA replication origins.Nature communications · 2025Article
- Regulation of DNA Topology in Archaea: State of the Art and Perspectives.Molecular microbiology · 2025Review
- Capturing chromosome conformation in Crenarchaea.Molecular microbiology · 2025Review
- Effects of genomic location on ectopic integration and gene expression of a reporter gene cassette inFrontiers in microbiology · 2025Article
- Archaeal DNA replication initiation: bridging LUCA's legacy and modern mechanisms.Frontiers in microbiology · 2025Article
- A vector system for single and tandem expression of cloned genes and multi-colour fluorescent tagging inMicrobiology (Reading, England) · 2024Article
- How Do Thermophiles Organize Their Genomes?Microbes and environments · 2024Review
- Form and function of archaeal genomes.Biochemical Society transactions · 2022Article
- Dissection of Functional Domains of Orc1-2, the Archaeal Global DNA Damage-Responsive Regulator.International journal of molecular sciences · 2022Article
- Chromosome organization affects genome evolution in Sulfolobus archaea.Nature microbiology · 2022Article
- Progress and Challenges in Archaeal Genetic Manipulation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Phenotypic Characterization ofFrontiers in microbiology · 2021Article
- Live Imaging of a Hyperthermophilic Archaeon Reveals Distinct Roles for Two ESCRT-III Homologs in Ensuring a Robust and Symmetric Division.Current biology : CB · 2020Article
- Article
- Analysis of the Archaeal ESCRT Apparatus.Methods in molecular biology (Clifton, N.J.) · 2019Article
- The DNA Methylome of the Hyperthermoacidophilic CrenarchaeonFrontiers in microbiology · 2018Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The "baby machine" provides a means of generating synchronized cultures of minimally perturbed cells. We describe the use of this technique to establish the key cell-cycle parameters of hyperthermophilic archaea of the genus Sulfolobus. The 3 DNA replication origins of Sulfolobus acidocaldarius were mapped by 2D gel analysis to near 0 (oriC2), 579 (oriC1), and 1,197 kb (oriC3) on the 2,226-kb circular genome, and we present a direct demonstration of their activity within the first few minutes of a synchronous cell cycle. We also detected X-shaped DNA molecules at the origins in log-phase cells, but these were not directly associated with replication initiation or ongoing chromosome replication in synchronized cells. Whole-genome marker frequency analyses of both synchronous and log-phase cultures showed that origin utilization was close to 100% for all 3 origins per round of replication. However, oriC2 was activated slightly later on average compared with oriC1 and oriC3. The DNA replication forks moved bidirectionally away from each origin at approximately 88 bp per second in synchronous culture. Analysis of the 3 Orc1/Cdc6 initiator proteins showed a uniformity of cellular abundance and origin binding throughout the cell cycle. In contrast, although levels of the MCM helicase were constant across the cell cycle, its origin localization was regulated, because it was strongly enriched at all 3 origins in early S phase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.