ArticleMolecular cell2008
Diversity-generating retroelement homing regenerates target sequences for repeated rounds of codon rewriting and protein diversification.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- RNA-templated diversity generation via reverse transcription and homologous recombination: an evolutionary hypothesis.Theory in biosciences = Theorie in den Biowissenschaften · 2026Article
- High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification.PLoS genetics · 2026Article
- Targeted protein evolution in the gut microbiome by diversity-generating retroelements.Science (New York, N.Y.) · 2025Article
- How and when organisms edit their own genomes.Nature genetics · 2025Review
- Incomplete lytic cycle of a widespread Bacteroides bacteriophage leads to the formation of defective viral particles.PLoS biology · 2025Article
- Article
- Targeted protein evolution in the gut microbiome by diversity-generating retroelements.bioRxiv : the preprint server for biology · 2024Article
- Targeted hypermutation of putative antigen sensors in multicellular bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- Discovery and characterization of the evolution, variation and functions of diversity-generating retroelements using thousands of genomes and metagenomes.BMC genomics · 2019Article
- Template-assisted synthesis of adenine-mutagenized cDNA by a retroelement protein complex.Nucleic acids research · 2018Article
- Diversity-generating retroelements: natural variation, classification and evolution inferred from a large-scale genomic survey.Nucleic acids research · 2018Article
- DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Retroelement-guided protein diversification abounds in vast lineages of Bacteria and Archaea.Nature microbiology · 2017Article
- Genomic and Metagenomic Analysis of Diversity-Generating Retroelements Associated with Treponema denticola.Frontiers in microbiology · 2016Article
- Regulatory RNAs in photosynthetic cyanobacteria.FEMS microbiology reviews · 2015Review
- Targeted diversity generation by intraterrestrial archaea and archaeal viruses.Nature communications · 2015Article
- Diversity-generating Retroelements in Phage and Bacterial Genomes.Microbiology spectrum · 2014Review
- The primary transcriptome of the marine diazotroph Trichodesmium erythraeum IMS101.Scientific reports · 2014Article
- Bacterial genome instability.Microbiology and molecular biology reviews : MMBR · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Diversity-generating retroelements (DGRs) introduce vast amounts of sequence diversity into target genes. During mutagenic homing, adenine residues are converted to random nucleotides in a unidirectional, reverse transcriptase-dependent transposition process from a donor template repeat (TR) to a recipient variable repeat (VR). Using a Bordetella bacteriophage DGR as a model, we demonstrate that homing occurs through a TR-containing RNA intermediate and is RecA independent. Marker transfer studies show that cDNA integration at the 3' end of VR occurs within a (G/C)(14) element, and deletion analysis demonstrates that the reaction is independent of 5' end cDNA integration. cDNA integration at the 5' end of VR requires only short stretches of sequence homology. We propose that homing occurs through a unique target DNA-primed reverse transcription mechanism that precisely regenerates target sequences. This nonproliferative "copy and replace" mechanism enables repeated rounds of protein diversification and optimization of ligand-receptor interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.