ArticleJournal of clinical microbiology1991
Isolation of a strain-specific Entamoeba histolytica cDNA clone.
Article in Journal of clinical microbiology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 49 citations in OpenAlex.
- Review
- Molecular epidemiology of amebiasis.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2008Article
- Laboratory diagnostic techniques for Entamoeba species.Clinical microbiology reviews · 2007Review
- Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area.Journal of clinical microbiology · 2002Article
- Isolation and characterization of polymorphic DNA from Entamoeba histolytica.Journal of clinical microbiology · 2001Article
- Progress towards development of a vaccine for amebiasis.Clinical microbiology reviews · 1997Review
- Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA.Epidemiology and infection · 1995Article
- DNA probes and PCR for diagnosis of parasitic infections.Clinical microbiology reviews · 1995Review
- A variable DNA region of Entamoeba histolytica is expressed in several transcripts which differ in genetically related clones.Molecular & general genetics : MGG · 1993Article
- Analysis of pathogenicity by restriction-endonuclease digestion of amplified genomic DNA of Entamoeba histolytica isolated in Pernambuco, Brazil.Parasitology research · 1992Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality worldwide. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. A cDNA library was constructed by using poly(A)+ RNA isolated from an axenic strain of E. histolytica, HM1:IMSS, which expresses a pathogenic isoenzyme pattern (zymodeme). Differential screening of the library yielded a strain-specific 3' polyadenylated cDNA clone, C2, possessing nine 26-nucleotide tandem repeats. RNA and DNA transfer blot analysis of four axenic strains of E. histolytica possessing the same pathogenic zymodeme revealed that the gene is present and expressed in pathogenic E. histolytica HM1:IMSS and 200:NIH but is not present in pathogenic strains HK-9 and Rahman. In addition, Southern blot analysis using the C2 clone showed heterogeneity of genomic organization between HM1:IMSS and 200:NIH. DNA dot blot hybridization analysis demonstrated that cDNA clone C2 was also able to distinguish axenically cultured E. histolytica strains possessing pathogenic zymodemes from those possessing nonpathogenic zymodemes and could detect as few as 100 amebic trophozoites. We conclude that C2 is a strain-specific E. histolytica cDNA clone that, in conjunction with other E. histolytica-specific probes, could serve as a useful epidemiologic tool.
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