ArticleAnalytical chemistry2008
Carbon nanotubes with enhanced chemiluminescence immunoassay for CCD-based detection of Staphylococcal enterotoxin B in food.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 90 citations in OpenAlex.
- Review
- Discovering Facet-Dependent Formation Kinetics of Key Intermediates in Electrochemical Ammonia Oxidation by a Electrochemiluminescence Active Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Recent Applications and Future Perspectives of Chemiluminescent and Bioluminescent Imaging Technologies.Chemical & biomedical imaging · 2023Review
- Endotoxin in Sepsis: Methods for LPS Detection and the Use of Omics Techniques.Diagnostics (Basel, Switzerland) · 2022Review
- Chemiluminescence and Bioluminescence Imaging for Biosensing and Therapy:Theranostics · 2019Review
- A Review on Passive and Integrated Near-Field Microwave Biosensors.Biosensors · 2017Review
- Elucidating endotoxin-biomolecule interactions with FRET: extending the frontiers of their supramolecular complexation.Journal of biological methods · 2017Review
- High-throughput living cell-based optical biosensor for detection of bacterial lipopolysaccharide (LPS) using a red fluorescent protein reporter system.Scientific reports · 2016Article
- Review
- Improving the Sensitivity and Functionality of Mobile Webcam-Based Fluorescence Detectors for Point-of-Care Diagnostics in Global Health.Diagnostics (Basel, Switzerland) · 2016Review
- Electrical percolation based biosensors.Methods (San Diego, Calif.) · 2013Article
- Image stacking approach to increase sensitivity of fluorescence detection using a low cost complementary metal-oxide-semiconductor (CMOS) webcam.Sensors and actuators. B, Chemical · 2012Article
- Lab-on-a-chip pathogen sensors for food safety.Sensors (Basel, Switzerland) · 2012Review
- An automated point-of-care system for immunodetection of staphylococcal enterotoxin B.Analytical biochemistry · 2011Article
- A simple 96 well microfluidic chip combined with visual and densitometry detection for resource-poor point of care testing.Sensors and actuators. B, Chemical · 2011Article
- Electrical percolation-based biosensor for real-time direct detection of staphylococcal enterotoxin B (SEB).Biosensors & bioelectronics · 2010Article
- Biological semiconductor based on electrical percolation.Analytical chemistry · 2010Article
- Gold nanoparticle-based enhanced chemiluminescence immunosensor for detection of Staphylococcal Enterotoxin B (SEB) in food.International journal of food microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Enhanced chemiluminescence (ECL) detection can significantly enhance the sensitivity of immunoassays but often requires expensive and complex detectors. The need for these detectors limits broader use of ECL in immunoassay applications. To make ECL more practical for immunoassays, we utilize a simple cooled charge-coupled device (CCD) detector combined with carbon nanotubes (CNTs) for primary antibody immobilization to develop a simple and portable point-of-care immunosensor. This combination of ECL, CNT, and CCD detector technologies is used to improve the detection of Staphylococcal enterotoxin B (SEB) in food. Anti-SEB primary antibodies were immobilized onto the CNT surface, and the antibody-nanotube mixture was immobilized onto a polycarbonate surface. SEB was then detected by an ELISA assay on the CNT-polycarbonate surface with an ECL assay. SEB in buffer, soy milk, apple juice, and meat baby food was assayed with a LOD of 0.01 ng/mL using our CCD detector, a level similar to the detection limit obtained with a fluorometric detector when using the CNTs. This level is far more sensitive than the conventional ELISA, which has a LOD of approximately 1 ng/mL. Our simple, versatile, and inexpensive point-of-care immunosensor combined with the CNT-ECL immunoassay method described in this work can also be used to simplify and increase sensitivity for many other types of diagnostics and detection assays.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.