ArticleHistochemistry and cell biology2009
Myocilin promotes substrate adhesion, spreading and formation of focal contacts in podocytes and mesangial cells.
Article in Histochemistry and cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- High throughput functional profiling of genes at intraocular pressure loci reveals distinct networks for glaucoma.Human molecular genetics · 2024Article
- Identification of key genes for atherosclerosis in different arterial beds.Scientific reports · 2024Article
- YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Myocilin misfolding and glaucoma: A 20-year update.Progress in retinal and eye research · 2023Review
- Transgenic Overexpression of Myocilin Leads to Variable Ocular Anterior Segment and Retinal Alterations Associated with Extracellular Matrix Abnormalities in Adult Zebrafish.International journal of molecular sciences · 2022Article
- Prostate Cancer Secretome and Membrane Proteome fromInternational journal of molecular sciences · 2022Article
- Proteomic characterization of the normal human medial meniscus body using data-independent acquisition mass spectrometry.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Identification of myocilin as a blood plasma protein and analysis of its role in leukocyte adhesion to endothelial cell monolayers.PloS one · 2018Article
- Structure and Misfolding of the Flexible Tripartite Coiled-Coil Domain of Glaucoma-Associated Myocilin.Structure (London, England : 1993) · 2017Article
- Glaucomatous MYOC mutations activate the IL-1/NF-κB inflammatory stress response and the glaucoma marker SELE in trabecular meshwork cells.Molecular vision · 2015Article
- NFATc1 activity regulates the expression of myocilin induced by dexamethasone.Experimental eye research · 2015Article
- Olfactomedin proteins: central players in development and disease.Frontiers in cell and developmental biology · 2014Review
- A molecular mechanism for glaucoma: endoplasmic reticulum stress and the unfolded protein response.Trends in molecular medicine · 2013Review
- The glaucoma-associated olfactomedin domain of myocilin is a novel calcium binding protein.The Journal of biological chemistry · 2012Article
- Amyloid fibril formation by the glaucoma-associated olfactomedin domain of myocilin.Journal of molecular biology · 2012Article
- Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: a microenvironment predisposed to tubal ectopic pregnancy.Laboratory investigation; a journal of technical methods and pathology · 2012Article
- Establishment of inducible wild type and mutant myocilin-GFP-expressing RGC5 cell lines.PloS one · 2012Article
- Myocilin, a glaucoma-associated protein, promotes cell migration through activation of integrin-focal adhesion kinase-serine/threonine kinase signaling pathway.Journal of cellular physiology · 2011Article
- Molecular chaperone function for myocilin.Investigative ophthalmology & visual science · 2011Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myocilin, a secreted glycoprotein of the olfactomedin family, is constitutively expressed in podocytes of the rat kidney and induced in mesangial cells during mesangioproliferative glomerulonephritis. As myocilin has been found to be associated with fibrillar components of the extracellular matrix, and adhesive properties have been shown for other members of the olfactomedin family, we hypothesized that myocilin might play a role in cell-matrix interactions in the glomerulus. To elucidate functional properties of myocilin, recombinant myocilin was expressed in 293 EBNA cells and purified by Ni-chelate and heparin chromatography. Culture plates were coated with myocilin, and primary rat mesangial cells and cells from an immortal murine podocyte cell line were seeded onto the plates in serum free conditions. Both cell types showed concentration-dependant attachment to myocilin, an effect that was statistically significant and could be blocked with specific antibodies. When compared to equal amounts of fibronectin or collagen 1, myocilin was less effective in promoting substrate adhesion. Synergistic effects in substrate adhesion were observed when myocilin was added to low concentrations of fibronectin. Twenty-five percent of cells that had attached to myocilin substrates showed spreading and expressed focal contacts which were labeled by vinculin/phalloidin staining. Comparable findings were observed when human or murine trabecular meshwork cells were seeded on myocilin substrates. Adhesive properties of myocilin required multimer formation, and were not observed when culture plates were coated with a C-terminal fragment of myocilin, containing the olfactomedin domain. We conclude that myocilin promotes substrate adhesion of podocytes and mesangial cells, and might contribute to cell-matrix adhesion of both cell types in vivo.
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