ReviewThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2009
A guide to the perplexed on the specificity of antibodies.
Review in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
84 citing papers in PubMed.
- Reassessment of the roles of coronin proteins as actin effectors and in signaling.PLoS biology · 2026Article
- Standardization of Immunohistochemistry Using an Anti-WASLB Antibody in the Skeletal Muscle of Teleost Fish.Current protocols · 2026Article
- Are we there yet with XNA aptamers?RSC advances · 2026Review
- Calretinin and Parvalbumin Trapping of TDP43 and XRCC1 Instructs Neocortical Interneuron Death in Neonatal Hypoxic-Ischemic Encephalopathy.Biomolecules · 2026Article
- January in focus in HCB: antibody validation.Histochemistry and cell biology · 2026Article
- De novo protein design: a transformative frontier in clinical protein applications.Journal of translational medicine · 2026Review
- June in focus in HCB.Histochemistry and cell biology · 2025Article
- Autophagy Analysis: A Step-by-Step Simple Practical Guide for Immunofluorescence and Western Blotting.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Antibody characterization is critical to enhance reproducibility in biomedical research.eLife · 2024Review
- TORSEL, a 4EBP1-based mTORC1 live-cell sensor, reveals nutrient-sensing targeting by histone deacetylase inhibitors.Cell & bioscience · 2024Article
- Article
- AB-Gen: Antibody Library Design with Generative Pre-trained Transformer and Deep Reinforcement Learning.Genomics, proteomics & bioinformatics · 2023Article
- Characterization of Antibodies against Receptor Activity-Modifying Protein 1 (RAMP1): A Cautionary Tale.International journal of molecular sciences · 2022Article
- PACInternational journal of molecular sciences · 2022Article
- Epitope Fine Mapping by Mass Spectrometry: Investigations of Immune Complexes Consisting of Monoclonal Anti-HpTGEKP Antibody and Zinc Finger Protein Linker Phospho-Hexapeptides.Chembiochem : a European journal of chemical biology · 2022Article
- Article
- Neuronal internalization of immunoglobulin G injected into the mouse brain by a novel absorption strategy to avoid unwanted interaction with immune complex using centrifugal filtration.Histochemistry and cell biology · 2022Article
- Semi-automated validation and quantification of CTLA-4 in 90 different tumor entities using multiple antibodies and artificial intelligence.Laboratory investigation; a journal of technical methods and pathology · 2022Article
- Label2label: training a neural network to selectively restore cellular structures in fluorescence microscopy.Journal of cell science · 2022Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.