ArticleProteome science2008
SELDI-TOF-MS determination of hepcidin in clinical samples using stable isotope labelled hepcidin as an internal standard.
Article in Proteome science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 63 citations in OpenAlex.
- Trial
- Oral Iron Treatment Response and Predictors in Anaemic Adolescents and Adults with IBD: A Prospective Controlled Open-Label Trial.Journal of Crohn's & colitis · 2017Trial
- Hepcidin as a potential predictor for preoperative anemia treatment with intravenous iron-A retrospective pilot study.PloS one · 2018Article
- SRM-MS Method Development for Hepcidin-25 Peptide.Journal of analytical methods in chemistry · 2018Article
- Modulation of hepcidin to treat iron deregulation: potential clinical applications.Expert review of hematology · 2016Review
- Laboratory use of hepcidin in renal transplant recipients.Biochemia medica · 2016Review
- Clinical significance of C-reactive protein levels in predicting responsiveness to iron therapy in patients with inflammatory bowel disease and iron deficiency anemia.Digestive diseases and sciences · 2015Article
- Serum hepcidin-25 and response to intravenous iron in patients with non-dialysis chronic kidney disease.Journal of nephrology · 2015Article
- Hepcidin and iron homeostasis during pregnancy.Nutrients · 2014Review
- Regulation of cellular iron metabolism and its implications in lung cancer progression.Medical oncology (Northwood, London, England) · 2014Review
- Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids.Nanomedicine : nanotechnology, biology, and medicine · 2014Article
- Improved mass spectrometry assay for plasma hepcidin: detection and characterization of a novel hepcidin isoform.PloS one · 2013Article
- Hemodiafiltration improves plasma 25-hepcidin levels: a prospective, randomized, blinded, cross-over study comparing hemodialysis and hemodiafiltration.Nephron extra · 2012Article
- A novel monoclonal antibody immunoassay for the detection of human serum hepcidin.Journal of gastroenterology · 2011Article
- High-throughput matrix-assisted laser desorption ionization-time-of-flight mass spectrometry method for quantification of hepcidin in human urine.Analytical chemistry · 2010Article
- Results of the first international round robin for the quantification of urinary and plasma hepcidin assays: need for standardization.Haematologica · 2009Article
- Hepcidin modulation in human diseases: from research to clinic.World journal of gastroenterology · 2009Review
- Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHepcidin is a 25-residue peptide hormone crucial to iron homeostasis. It is essential to measure the concentration of hepcidin in cells, tissues and body fluids to understand its mechanisms and roles in physiology and pathophysiology. With a mass of 2791 Da hepcidin is readily detectable by mass spectrometry and LC-ESI, MALDI and SELDI have been used to estimate systemic hepcidin concentrations by analysing serum or urine. However, peak heights in mass spectra may not always reflect concentrations in samples due to competition during binding steps and variations in ionisation efficiency. Thus the purpose of this study was to develop a robust assay for measuring hepcidin using a stable isotope labelled hepcidin spiking approach in conjunction with SELDI-TOF-MS.
resultsWe synthesised and re-folded hepcidin labelled with 13C/15N phenylalanine at position 9 to generate an internal standard for mass spectrometry experiments. This labelled hepcidin is 10 Daltons heavier than the endogenous peptides and does not overlap with the isotopic envelope of the endogenous hepcidin or other common peaks in human serum or urine mass spectra and can be distinguished in low resolution mass spectrometers. We report the validation of adding labelled hepcidin into serum followed by SELDI analysis to generate an improved assay for hepcidin.
conclusionWe demonstrate that without utilising a spiking approach the hepcidin peak height in SELDI spectra gives a good indication of hepcidin concentration. However, a stable isotope labelled hepcidin spiking approach provides a more robust assay, measures the absolute concentration of hepcidin and should facilitate inter-laboratory hepcidin comparisons.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.