ArticleBMC genomics2008
The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 53 citations in OpenAlex.
- Metabolic engineering strategies of Corynebacterium glutamicum for sulfur-containing amino acid production: current progress and future directions.World journal of microbiology & biotechnology · 2026Review
- Elucidating the Mechanism of Temporal Adaptation to Hydrogen Peroxide-Induced Oxidative Stress in Corynebacterium glutamicum.Microbial biotechnology · 2025Article
- ALE reveals a surprising link between [Fe-S] cluster formation, tryptophan biosynthesis and the potential regulatory protein TrpP in Corynebacterium glutamicum.BMC microbiology · 2025Article
- Systems-wide analysis of the ROK-family regulatory geneApplied and environmental microbiology · 2023Article
- Transcription factor OxyR regulates sulfane sulfur removal and L-cysteine biosynthesis inApplied and environmental microbiology · 2023Article
- Physiology and Transcriptional Analysis of (p)ppGpp-Related Regulatory Effects inFrontiers in microbiology · 2019Article
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics.Scientific reports · 2018Article
- Characterization of DNA Binding Sites of RokB, a ROK-Family Regulator from Streptomyces coelicolor Reveals the RokB Regulon.PloS one · 2016Article
- Transcriptional Regulation of the β-Type Carbonic Anhydrase Gene bca by RamA in Corynebacterium glutamicum.PloS one · 2016Article
- Transcription of two adjacent carbohydrate utilization gene clusters in Bifidobacterium breve UCC2003 is controlled by LacI- and repressor open reading frame kinase (ROK)-type regulators.Applied and environmental microbiology · 2014Article
- Protein S-mycothiolation functions as redox-switch and thiol protection mechanism in Corynebacterium glutamicum under hypochlorite stress.Antioxidants & redox signaling · 2014Article
- Visualization of imbalances in sulfur assimilation and synthesis of sulfur-containing amino acids at the single-cell level.Applied and environmental microbiology · 2013Article
- Comprehensive discovery and characterization of small RNAs in Corynebacterium glutamicum ATCC 13032.BMC genomics · 2013Article
- A novel type of N-acetylglutamate synthase is involved in the first step of arginine biosynthesis in Corynebacterium glutamicum.BMC genomics · 2013Article
- Article
- Complete genome sequence of Corynebacterium variabile DSM 44702 isolated from the surface of smear-ripened cheeses and insights into cheese ripening and flavor generation.BMC genomics · 2011Article
- The regulation of sulfur metabolism in Mycobacterium tuberculosis.PLoS pathogens · 2011Review
- Global regulation of the response to sulfur availability in the cheese-related bacterium Brevibacterium aurantiacum.Applied and environmental microbiology · 2011Article
- Three paralogous LysR-type transcriptional regulators control sulfur amino acid supply in Streptococcus mutans.Journal of bacteriology · 2010Article
- Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis.BMC genomics · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRegulation of sulphur metabolism in Corynebacterium glutamicum ATCC 13032 has been studied intensively in the last few years, due to its industrial as well as scientific importance. Previously, the gene cg0156 was shown to belong to the regulon of McbR, a global transcriptional repressor of sulphur metabolism in C. glutamicum. This gene encodes a putative ROK-type regulator, a paralogue of the activator of sulphonate utilisation, SsuR. Therefore, it is an interesting candidate for study to further the understanding of the regulation of sulphur metabolism in C. glutamicum.
resultsDeletion of cg0156, now designated cysR, results in the inability of the mutant to utilise sulphate and aliphatic sulphonates. DNA microarray hybridisations revealed 49 genes with significantly increased and 48 with decreased transcript levels in presence of the native CysR compared to a cysR deletion mutant. Among the genes positively controlled by CysR were the gene cluster involved in sulphate reduction, fpr2 cysIXHDNYZ, and ssuR. Gel retardation experiments demonstrated that binding of CysR to DNA depends in vitro on the presence of either O-acetyl-L-serine or O-acetyl-L-homoserine. Mapping of the transcription start points of five transcription units helped to identify a 10 bp inverted repeat as the possible CysR binding site. Subsequent in vivo tests proved this motif to be necessary for CysR-dependent transcriptional regulation.
conclusionCysR acts as the functional analogue of the unrelated LysR-type regulator CysB from Escherichia coli, controlling sulphide production in response to acceptor availability. In both bacteria, gene duplication events seem to have taken place which resulted in the evolution of dedicated regulators for the control of sulphonate utilisation. The striking convergent evolution of network topology indicates the strong selective pressure to control the metabolism of the essential but often toxic sulphur-containing (bio-)molecules.
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