ArticleGlycoconjugate journal2009
Specific oligomerization of the 5-HT1A receptor in the plasma membrane.
Article in Glycoconjugate journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- The interplay of serotonin 5-HT1A and 5-HT7 receptors in chronic stress.Journal of cell science · 2024Article
- Serotonin system in the human placenta - the knowns and unknowns.Frontiers in endocrinology · 2022Review
- The functional cooperation of 5-HTPharmacological reports : PR · 2020Article
- What Do We Really Know About 5-HTFrontiers in cellular neuroscience · 2016Review
- Interplay between serotonin 5-HT1A and 5-HT7 receptors in depressive disorders.CNS neuroscience & therapeutics · 2014Review
- The effects of chronic ethanol self-administration on hippocampal 5-HT1A receptors in monkeys.Drug and alcohol dependence · 2014Article
- Fluorescence correlation spectroscopy analysis of serotonin, adrenergic, muscarinic, and dopamine receptor dimerization: the oligomer number puzzle.Molecular pharmacology · 2013Article
- Functional significance of serotonin receptor dimerization.Experimental brain research · 2013Review
- Quantitative intensity-based FRET approaches--a comparative snapshot.Biophysical journal · 2012Review
- A palmitoylation switch mechanism regulates Rac1 function and membrane organization.The EMBO journal · 2012Article
- Homodimerization of the Src homology 3 domain of the calcium channel β-subunit drives dynamin-dependent endocytosis.The Journal of biological chemistry · 2011Article
- Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C-dependent regulation.The Journal of biological chemistry · 2011Article
- Förster resonance energy transfer evidence for lysozyme oligomerization in lipid environment.The journal of physical chemistry. B · 2010Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study we analyze the oligomerization of the 5-HT1A receptor within living cells at the sub-cellular level. Using a 2-excitation Förster Resonance Energy Transfer (FRET) method combined with spectral microscopy we are able to estimate the efficiency of energy transfer based on donor quenching as well as acceptor sensitization between CFP-and YFP-tagged 5-HT1A receptors at the plasma membrane. Through the analysis of the level of apparent FRET efficiency over the various relative amounts of donor and acceptor, as well as over a range of total surface expressions of the receptor, we verify the specific interaction of these receptors. Furthermore we study the role of acylation in this interaction through measurements of a palmitoylation-deficient 5-HT(1A) receptor mutant. Palmitoylation increases the tendency of a receptor to localize in lipid rich microdomains of the plasma membrane. This increases the effective surface density of the receptor and provides for a higher level of stochastic interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.