ArticleBrain cell biology2008
Imaging synaptic inhibition throughout the brain via genetically targeted Clomeleon.
Article in Brain cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- WNK-SPAK/OSR1 signaling pathway facilitates ictal activity via reduced neuronal chloride extrusion rate.bioRxiv : the preprint server for biology · 2026Article
- Timing of interventions to control neuronal chloride elevation in a model of neonatal seizures after hippocampal injury.Epilepsia · 2024Article
- Role of NKCC1 and KCC2 during hypoxia-induced neuronal swelling in the neonatal neocortex.Neurobiology of disease · 2023Article
- Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system.Experimental animals · 2020Article
- Rodent models for psychiatric disorders: problems and promises.Laboratory animal research · 2020Review
- Transient ischemia facilitates neuronal chloride accumulation and severity of seizures.Annals of clinical and translational neurology · 2018Article
- KCC2-dependent Steady-state Intracellular Chloride Concentration and pH in Cortical Layer 2/3 Neurons of Anesthetized and Awake Mice.Frontiers in cellular neuroscience · 2018Article
- Giant Depolarizing Potentials Trigger Transient Changes in the Intracellular ClFrontiers in cellular neuroscience · 2018Article
- A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence.Science advances · 2016Article
- Optogenetic Visualization of Presynaptic Tonic Inhibition of Cerebellar Parallel Fibers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Spines slow down dendritic chloride diffusion and affect short-term ionic plasticity of GABAergic inhibition.Scientific reports · 2016Article
- Disinhibition of olfactory bulb granule cells accelerates odour discrimination in mice.Nature communications · 2015Article
- Optogenetic tools for modulating and probing the epileptic network.Epilepsy research · 2015Review
- Anoctamin Calcium-Activated Chloride Channels May Modulate Inhibitory Transmission in the Cerebellar Cortex.PloS one · 2015Article
- Twenty years of fluorescence imaging of intracellular chloride.Frontiers in cellular neuroscience · 2014Review
- Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Transgenic mouse lines for non-invasive ratiometric monitoring of intracellular chloride.Frontiers in molecular neuroscience · 2013Article
- Calibration and functional analysis of three genetically encoded Cl(-)/pH sensors.Frontiers in molecular neuroscience · 2013Article
- K+-Cl- cotransporter-2 KCC2 in chicken cardiomyocytes.American journal of physiology. Cell physiology · 2012Article
- Columnar distribution of activity dependent gabaergic depolarization in sensorimotor cortical neurons.Molecular brain · 2012Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Here we survey a molecular genetic approach for imaging synaptic inhibition. This approach is based on measuring intracellular chloride concentration ([Cl(-)](i)) with the fluorescent chloride indicator protein, Clomeleon. We first describe several different ways to express Clomeleon in selected populations of neurons in the mouse brain. These methods include targeted viral gene transfer, conditional expression controlled by Cre recombination, and transgenesis based on the neuron-specific promoter, thy1. Next, we evaluate the feasibility of using different lines of thy1::Clomeleon transgenic mice to image synaptic inhibition in several different brain regions: the hippocampus, the deep cerebellar nuclei (DCN), the basolateral nucleus of the amygdala, and the superior colliculus (SC). Activation of hippocampal interneurons caused [Cl(-)](i) to rise transiently in individual postsynaptic CA1 pyramidal neurons. [Cl(-)](i) increased linearly with the number of electrical stimuli in a train, with peak changes as large as 4 mM. These responses were largely mediated by GABA receptors because they were blocked by antagonists of GABA receptors, such as GABAzine and bicuculline. Similar responses to synaptic activity were observed in DCN neurons, amygdalar principal cells, and collicular premotor neurons. However, in contrast to the hippocampus, the responses in these three regions were largely insensitive to antagonists of inhibitory neurotransmitter receptors. This indicates that synaptic activity can also cause Cl(-) influx through alternate pathways that remain to be identified. We conclude that Clomeleon imaging permits non-invasive, spatiotemporally precise recordings of [Cl(-)](i) in a large variety of neurons, and provides new opportunities for imaging synaptic inhibition and other forms of neuronal chloride signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.