ArticleApplied and environmental microbiology2008
Quantification of diatom and dinoflagellate biomasses in coastal marine seawater samples by real-time PCR.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Biofilm Source Shapes the Tolerance to Short-Term Ciprofloxacin Exposure.Environmental microbiology reports · 2026Article
- From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR.Molecular ecology resources · 2026Article
- Comparing DNA metabarcoding with light microscopy to identify eukaryotic phytoplankton in the Baltic Sea, Kattegat and Skagerrak.Scientific reports · 2026Article
- Phytoplankton Diversity in the Northern Adriatic Sea: Insights and Inconsistencies from Microscopy and Metabarcoding.Biology · 2026Article
- Standardization of A Method for the Detection of the Genus Prorocentrum, A Dinoflagellate Present in Harmful Algae Blooms (habs) in San Jorge Antofagasta Bay by Digital Droplet Pcr (ddPCR).Marine biotechnology (New York, N.Y.) · 2026Article
- A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response.Ecology and evolution · 2026Article
- Environmental drivers and key taxa shaping diatom-dinoflagellate ratios in eutrophic coastal waters.Marine life science & technology · 2026Article
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- A protozoan perspective on climate change and biosafety threats: differences in testate amoebae in lakes in forest-swamp and forest-steppe zones in Western Siberia.Applied and environmental microbiology · 2025Article
- Comparison of Relative and Absolute Abundance and Biomass of Freshwater Phytoplankton Taxa Using Metabarcoding and Microscopy.Ecology and evolution · 2025Article
- The protist community of the oligotrophic waters of the Gulf of Mexico is distinctly shaped by depth-specific physicochemical conditions during the warm season.FEMS microbiology ecology · 2025Article
- The Ribosomal Operon Database: A Full-Length rDNA Operon Database Derived From Genome Assemblies.Molecular ecology resources · 2025Article
- Vulnerability of Arctic Ocean microbial eukaryotes to sea ice loss.Scientific reports · 2024Article
- Picophytoplankton prevail year-round in the Elbe estuary.Plant-environment interactions (Hoboken, N.J.) · 2024Article
- Prevalence and Preferred Niche of Small Eukaryotes with Mixotrophic Potentials in the Global Ocean.Microorganisms · 2024Article
- Rich diversity and active spatial-temporal dynamics ofISME communications · 2024Article
- Taxon-specific contributions of microeukaryotes to biological carbon pump in the Oyashio region.ISME communications · 2024Article
- Spatiotemporal assembly and functional composition of planktonic microeukaryotic communities along productivity gradients in a subtropical lake.Frontiers in microbiology · 2024Article
- Mock community experiments can inform on the reliability of eDNA metabarcoding data: a case study on marine phytoplankton.Scientific reports · 2023Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Two real-time PCR assays targeting the small-subunit (SSU) ribosomal DNA (rDNA) were designed to assess the proportional biomass of diatoms and dinoflagellates in marine coastal water. The reverse primer for the diatom assay was designed to be class specific, and the dinoflagellate-specific reverse primer was obtained from the literature. For both targets, we used universal eukaryotic SSU rDNA forward primers. Specificity was confirmed by using a BLAST search and by amplification of cultures of various phytoplankton taxa. Reaction conditions were optimized for each primer set with linearized plasmids from cloned SSU rDNA fragments. The number of SSU rDNA copies per cell was estimated for six species of diatoms and nine species of dinoflagellates; these were significantly correlated to the biovolumes of the cells. Nineteen field samples were collected along the Swedish west coast and subjected to the two real-time PCR assays. The linear regression of the proportion of SSU rDNA copies of dinoflagellate and diatom origin versus the proportion of dinoflagellate and diatom biovolumes or biomass per liter was significant. For diatoms, linear regression of the number of SSU rDNA copies versus biovolume or biomass per liter was significant, but no such significant correlation was detected in the field samples for dinoflagellates. The method described will be useful for estimating the proportion of dinoflagellate versus diatom biovolume or biomass and the absolute diatom biovolume or biomass in various aquatic disciplines.
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