ArticleApplied and environmental microbiology2008
Origin and diversity of metabolically active gut bacteria from laboratory-bred larvae of Manduca sexta (Sphingidae, Lepidoptera, Insecta).
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 90 citations in OpenAlex.
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- Microbial Symbiosis in Lepidoptera: Analyzing the Gut Microbiota for Sustainable Pest Management.Biology · 2025Review
- Significant variations of bacterial communities among the developmental stages of Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae).Scientific reports · 2025Article
- Bacterial communities inHeliyon · 2025Article
- The Diversity ofInsects · 2024Article
- The effect of diet composition on the diversity of active gut bacteria and on the growth of Spodoptera exigua (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2024Article
- Comparative analysis ofPeerJ · 2024Article
- Bacterial Communities Associated with Houseflies (Microorganisms · 2023Article
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- Composition and diversity of gut microbiota across developmental stages ofFrontiers in microbiology · 2023Article
- High-throughput screening of caterpillars as a platform to study host-microbe interactions and enteric immunity.Nature communications · 2022Article
- Effect of Diet on the Midgut Microbial Composition and Host Immunity of the Fall Armyworm,Biology · 2022Article
- Fall Armyworm Gut Bacterial Diversity Associated with Different Developmental Stages, Environmental Habitats, and Diets.Insects · 2022Article
- Article
- Comparison of Gut Bacterial Communities of Fall Armyworm (International journal of molecular sciences · 2021Article
- Bacterial Microbiota of Field-CollectedMicroorganisms · 2021Article
- Contribution of sample processing to gut microbiome analysis in the model Lepidoptera, silkwormComputational and structural biotechnology journal · 2021Article
- Article
- Antimicrobial resistance and genetic relationships of enterococci from siblings and non-siblingsPeerJ · 2020Article
- Gut bacterial and fungal communities of the domesticated silkworm (Bombyx mori) and wild mulberry-feeding relatives.The ISME journal · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultivation-independent analyses based on genetic profiling of partial bacterial 16S rRNA genes by PCR-single-strand conformation polymorphism (PCR-SSCP), reverse transcriptase (RT)-PCR-SSCP of the 16S rRNA itself, and stable isotope probing (SIP), followed by RT-PCR-SSCP, were applied to characterize the diversity of metabolically active bacteria in the larval gut of Manduca sexta bred on tobacco leaves under greenhouse conditions. For SIP, hatching larvae were fed with leaves from tobacco plants grown in a (13)CO(2)-enriched atmosphere. Dominant SSCP bands were sequenced and phylogenetically analyzed. Only one major gut colonizer, an Enterococcus relative, was detected; it occurred in the heavy RNA fraction, demonstrating its metabolic activity, and it originated from eggs, where its metabolic activity was also indicated by rRNA-based SSCP profiles. In contrast, a Citrobacter sedlakii relative was detected on eggs by DNA-SSCP, but rRNA-SSCP and SIP-rRNA-SSCP were negative, suggesting that these bacterial cells were inactive. A Burkholderia relative was dominant and metabolically active on the tobacco leaves but inactive inside the gut, where it was also quantitatively reduced, as suggested by lower band intensities in the DNA-based SSCP profiles. SIP-RNA-SSCP detected another metabolically active gut bacterium (Enterobacter sp.) and more bacteria in the light RNA fraction, indicating low or no metabolic activity of the latter inside the gut. We conclude that the larval gut supported only a low diversity of metabolically active bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.