ArticleApplied and environmental microbiology2008
Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed, 194 citations in OpenAlex.
- Article
- MtrAB activates ectoine production and triggers sporulation in response to osmotic stress inMicrobiology (Reading, England) · 2026Article
- Salt supplementation-induced metabolic reprogramming inmSystems · 2026Article
- Bacterial Espresso: Evaluating Accelerated Solvent Extraction for Selected Metabolite Recovery fromJournal of natural products · 2026Article
- Biosynthesis and import of the cytoprotective extremolytes ectoine and hydroxyectoine in the phylumFrontiers in microbiology · 2026Article
- Physiological Insights into Enhanced Epsilon-Poly-l-Lysine Production Induced by Extract Supplement from HeterogeneousMicroorganisms · 2025Article
- Eliciting Clavulanic Acid Biosynthesis: The Impact ofMetabolites · 2025Article
- Plant Growth-Promoting Effect and Complete Genomic Sequence Analysis of the Beneficial RhizosphereMicroorganisms · 2025Article
- Organic and inorganic fertilizers modulate the response of the soil microbiome to salinity stress.Frontiers in microbiology · 2025Article
- Beyond methane consumption: exploring the potential of methanotrophic bacteria to produce secondary metabolites.ISME communications · 2025Article
- The role of two major nucleoid-associated proteins in Streptomyces, HupA and HupS, in stress survival and gene expression regulation.Microbial cell factories · 2024Article
- Role of Carbon, Nitrogen, Phosphate and Sulfur Metabolism in Secondary Metabolism Precursor Supply inMicroorganisms · 2024Review
- Biosynthesis of novel desferrioxamine derivatives requires unprecedented crosstalk between separate NRPS-independent siderophore pathways.Applied and environmental microbiology · 2024Article
- Glucosylglycerol phosphorylase, a potential novel pathway of microbial glucosylglycerol catabolism.Applied microbiology and biotechnology · 2024Article
- Comparative genomics of the extremophileFrontiers in fungal biology · 2024Article
- Metabolic engineering of Halomonas campaniensis strain XH26 to remove competing pathways to enhance ectoine production.Scientific reports · 2023Article
- Review
- The phylogeny and metabolic potentials of anFrontiers in microbiology · 2023Article
- Research progress on GlnR-mediated regulation in Actinomycetes.Frontiers in microbiology · 2023Review
- High-efficiency production of 5-hydroxyectoine using metabolically engineered Corynebacterium glutamicum.Microbial cell factories · 2022Article
49 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptomyces coelicolor A3(2) synthesizes ectoine and 5-hydroxyectoine upon the imposition of either salt (0.5 M NaCl) or heat stress (39 degrees C). The cells produced the highest cellular levels of these compatible solutes when both stress conditions were simultaneously imposed. Protection against either severe salt (1.2 M NaCl) or heat stress (39 degrees C) or a combination of both environmental cues could be accomplished by adding low concentrations (1 mM) of either ectoine or 5-hydroxyectoine to S. coelicolor A3(2) cultures. The best salt and heat stress protection was observed when a mixture of ectoine and 5-hydroxyectoine (0.5 mM each) was provided to the growth medium. Transport assays with radiolabeled ectoine demonstrated that uptake was triggered by either salt or heat stress. The most effective transport and accumulation of [(14)C]ectoine by S. coelicolor A3(2) were achieved when both environmental cues were simultaneously applied. Our results demonstrate that the accumulation of the compatible solutes ectoine and 5-hydroxyectoine allows S. coelicolor A3(2) to fend off the detrimental effects of both high salinity and high temperature on cell physiology. We also characterized the enzyme (EctD) required for the synthesis of 5-hydroxyectoine from ectoine, a hydroxylase of the superfamily of the non-heme-containing iron(II)- and 2-oxoglutarate-dependent dioxygenases (EC 1.14.11). The gene cluster (ectABCD) encoding the enzymes for ectoine and 5-hydroxyectoine biosynthesis can be found in the genome of S. coelicolor A3(2), Streptomyces avermitilis, Streptomyces griseus, Streptomyces scabiei, and Streptomyces chrysomallus, suggesting that these compatible solutes play an important role as stress protectants in the genus Streptomyces.
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