ArticleBiophysical journal2009
Sphingomyelinase-induced domain shape relaxation driven by out-of-equilibrium changes of composition.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The Effect of Ceramide Ratio on the Membrane Curvature of Mimetic Models of Matrix Vesicles.ACS physical chemistry Au · 2025Article
- Review
- Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls.International journal of molecular sciences · 2021Article
- Interplay Between Plasma Membrane Lipid Alteration, Oxidative Stress and Calcium-Based Mechanism for Extracellular Vesicle Biogenesis From Erythrocytes During Blood Storage.Frontiers in physiology · 2020Article
- Review
- The many faces (and phases) of ceramide and sphingomyelin II - binary mixtures.Biophysical reviews · 2017Review
- Lipid Phase Separation and Protein-Ganglioside Clustering in Supported Bilayers Are Induced by Photorelease of Ceramide.Soft matter · 2013Article
- Photouncaging of ceramides promotes reorganization of liquid-ordered domains in supported lipid bilayers.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Effects of sphingomyelin headgroup size on interactions with ceramide.Biophysical journal · 2013Article
- Phase-field model for the morphology of monolayer lipid domains.The European physical journal. E, Soft matter · 2012Article
- Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains.Journal of lipid research · 2011Article
- Implication of sphingomyelin/ceramide molar ratio on the biological activity of sphingomyelinase.Biophysical journal · 2010Article
- End-products diacylglycerol and ceramide modulate membrane fusion induced by a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa.Biochimica et biophysica acta · 2010Article
- Coexistence of immiscible mixtures of palmitoylsphingomyelin and palmitoylceramide in monolayers and bilayers.Biophysical journal · 2009Article
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5 authors.
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Abstract
Sphingomyelinase (SMase)-induced ceramide (Cer)-enriched domains in a lipid monolayer are shown to result from an out-of-equilibrium situation. This is induced by a change of composition caused by the enzymatic production of Cer in a sphingomyelin (SM) monolayer that leads to a fast SM/Cer demixing into a liquid-condensed (LC), Cer-enriched and a liquid-expanded, SM-enriched phases. The morphological evolution and kinetic dependence of Cer-enriched domains is studied under continuous observation by epifluorescence microscopy. Domain shape annealing is observed from branched to rounded shapes after SMase activity quenching by EDTA, with a decay halftime of approximately 10 min. An out-of-equilibrium fast domain growth is not the determinant factor for domain morphology. Domain shape rearrangement in nearly equilibrium conditions result from the counteraction of intradomain dipolar repulsion and line tension, according to McConnell's shape transition theory. Phase separation causes a transient compositional overshoot within the LC phase that implies an increased out-of-equilibrium enrichment of Cer into the LC domains. As a consequence, higher intradomain repulsion leads to transient branched structures that relax to rounded shapes by lowering the proportion of Cer in the domain to equilibrium values. The fast action of SMase can be taken as a compositional perturbation that brings about important consequences for the surface organization.
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