ArticleChemistry & biology2008
Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora.
Article in Chemistry & biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 109 citations in OpenAlex.
- Trial
- Degradation of mucinbioRxiv : the preprint server for biology · 2026Article
- Dual-Activity Mannosyltransferase Phosphorylases in Family 130 of Glycoside Hydrolases.ACS catalysis · 2026Article
- Bespoke Activity-Based Probes Reveal that theJournal of the American Chemical Society · 2025Article
- Acarbose impairs gutmBio · 2024Article
- Determinants of raffinose family oligosaccharide use inJournal of bacteriology · 2024Article
- Catalytic Mode and Product Specificity of an α-Agarase Reveal Its Direct Catalysis for the Production of Agarooligosaccharides.Foods (Basel, Switzerland) · 2024Article
- Determinants of raffinose family oligosaccharide use inbioRxiv : the preprint server for biology · 2024Article
- Acarbose Impairs GutbioRxiv : the preprint server for biology · 2024Article
- Multifaceted roles of plant glycosyl hydrolases during pathogen infections: more to discover.Planta · 2024Review
- The effect of barium and strontium on activity of glucoamylase QsGH97a from Qipengyuania seohaensis SW-135.Scientific reports · 2023Article
- Characterizing the Effect of Amylase Inhibitors on Maltodextrin Metabolism by Gut Bacteria Using Fluorescent Glycan Labeling.ACS chemical biology · 2023Article
- Identification and Characterization of Dextran α-1,2-Debranching Enzyme fromJournal of applied glycoscience · 2023Article
- Three New Acrylic Acid Derivatives fromMolecules (Basel, Switzerland) · 2022Article
- Biological Evaluation, Molecular Docking Analyses, and ADME Profiling of Certain New Quinazolinones as Anti-colorectal Agents.ACS omega · 2022Article
- How microbial glycosyl hydrolase activity in the gut mucosa initiates microbial cross-feeding.Glycobiology · 2022Review
- Mechanism-Based Pharmacokinetic Model for the Deglycosylation Kinetics of 20(S)-Ginsenosides Rh2.Frontiers in pharmacology · 2022Article
- A Novel Family of RNA-Binding Proteins Regulate Polysaccharide Metabolism inJournal of bacteriology · 2021Article
- A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation.Nature microbiology · 2018Article
- Chitinase: diversity, limitations, and trends in engineering for suitable applications.Bioscience reports · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Enzymatic cleavage of the glycosidic bond yields products in which the anomeric configuration is either retained or inverted. Each mechanism reflects the dispositions of the enzyme functional groups; a facet of which is essentially conserved in 113 glycoside hydrolase (GH) families. We show that family GH97 has diverged significantly, as it contains both inverting and retaining alpha-glycosidases. This reflects evolution of the active center; a glutamate acts as a general base in inverting members, exemplified by Bacteroides thetaiotaomicron alpha-glucosidase BtGH97a, whereas an aspartate likely acts as a nucleophile in retaining members. The structure of BtGH97a and its complexes with inhibitors, coupled to kinetic analysis of active-site variants, reveals an unusual calcium ion dependence. 1H NMR analysis shows an inversion mechanism for BtGH97a, whereas another GH97 enzyme from B. thetaiotaomicron, BtGH97b, functions as a retaining alpha-galactosidase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.