ArticleJournal of the American Chemical Society2008
Unnatural substrate repertoire of A, B, and X family DNA polymerases.
Article in Journal of the American Chemical Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Recognition of unnatural base pairs by a eukaryotic DNA polymerase enables universal sequencing of an expanded genetic alphabet.Nucleic acids research · 2026Article
- FASTAptameR 2.0: A web tool for combinatorial sequence selections.Molecular therapy. Nucleic acids · 2022Article
- Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates.Frontiers in molecular biosciences · 2019Review
- The expanded genetic alphabet.Angewandte Chemie (International ed. in English) · 2015Review
- Functionalized tricyclic cytosine analogues provide nucleoside fluorophores with improved photophysical properties and a range of solvent sensitivities.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- Systematic exploration of a class of hydrophobic unnatural base pairs yields multiple new candidates for the expansion of the genetic alphabet.Nucleic acids research · 2014Article
- Major groove substituents and polymerase recognition of a class of predominantly hydrophobic unnatural base pairs.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Article
- Site-specific labeling of DNA and RNA using an efficiently replicated and transcribed class of unnatural base pairs.Journal of the American Chemical Society · 2011Article
- Solution structure, mechanism of replication, and optimization of an unnatural base pair.Chemistry (Weinheim an der Bergstrasse, Germany) · 2010Article
- Non-natural nucleotides as probes for the mechanism and fidelity of DNA polymerases.Biochimica et biophysica acta · 2010Review
- DNA damage and interstrand cross-link formation upon irradiation of aryl iodide C-nucleotide analogues.The Journal of organic chemistry · 2010Article
- Nucleotide Analogues as Probes for DNA and RNA Polymerases.Current protocols in chemical biology · 2010Article
- Major groove derivatization of an unnatural base pair.Chembiochem : a European journal of chemical biology · 2009Article
- Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by Deep Vent (exo- ) DNA polymerase.Nucleic acids research · 2009Article
- The effects of unnatural base pairs and mispairs on DNA duplex stability and solvation.Nucleic acids research · 2009Article
- Transcription of an expanded genetic alphabet.Journal of the American Chemical Society · 2009Article
- Optimization of an unnatural base pair toward natural-like replication.Journal of the American Chemical Society · 2009Article
- Efficient replication bypass of size-expanded DNA base pairs in bacterial cells.Angewandte Chemie (International ed. in English) · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
As part of an effort to develop unnatural base pairs that are stable and replicable in DNA, we examined the ability of five different polymerases to replicate DNA containing four different unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs. The unnatural pairs were developed based on intensive studies using the Klenow fragment of DNA polymerase I from E. coli (Kf) and found to be recognized to varying degrees. The five additional polymerases characterized here include family A polymerases from bacteriophage T7 and Thermus aquaticus, family B polymerases from Thermococcus litoralis and Thermococcus 9(o)N-7, and the family X polymerase, human polymerase beta. While we find that some aspects of unnatural base pair recognition are conserved among the polymerases, for example, the pair formed between two d3FB nucleotides is typically well recognized, the detailed recognition of most of the unnatural base pairs is generally polymerase dependent. In contrast, we find that the pair formed between d5SICS and dMMO2 is generally well recognized by all of the polymerases examined, suggesting that the determinants of efficient and general recognition are contained within the geometric and electronic structure of these unnatural nucleobases themselves. The data suggest that while the d3FB:d3FB pair is sufficiently well recognized by several of the polymerases for in vitro applications, the d5SICS:dMMO2 heteropair is likely uniquely promising for in vivo use. T7-mediated replication is especially noteworthy due to strong mispair discrimination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.