ArticleGenetics2008
A and C genome distinction and chromosome identification in brassica napus by sequential fluorescence in situ hybridization and genomic in situ hybridization.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 86 citations in OpenAlex.
- Stable resynthesized Brassica napus lines show similar meiotic behaviour to established B. napus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Article
- Novel Brassica hybrids with different resistance toOpen life sciences · 2022Article
- Development of B. carinata with super-high erucic acid content through interspecific hybridization.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Subgenome Discrimination inCells · 2021Article
- A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus.The New phytologist · 2021Article
- Chromosome inheritance and meiotic stability in allopolyploid Brassica napus.G3 (Bethesda, Md.) · 2021Article
- Insights Into the Molecular Evolution of AT-Hook Motif Nuclear Localization Genes inFrontiers in plant science · 2021Article
- Chromosome-nuclear envelope tethering - a process that orchestrates homologue pairing during plant meiosis?Journal of cell science · 2020Review
- Article
- DetectingG3 (Bethesda, Md.) · 2018Article
- FANCM Limits Meiotic Crossovers in Brassica Crops.Frontiers in plant science · 2018Article
- Polyploidy and the relationship between leaf structure and function: implications for correlated evolution of anatomy, morphology, and physiology in Brassica.BMC plant biology · 2017Article
- Gene conversion events and variable degree of homogenization of rDNA loci in cultivars of Brassica napus.Annals of botany · 2017Article
- Rapid genomic changes in allopolyploids of Carassius auratus red var. (♀) × Megalobrama amblycephala (♂).Scientific reports · 2016Article
- A modified method for preparing meiotic chromosomes based on digesting pollen mother cells in suspension.Molecular cytogenetics · 2015Article
- Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers.Journal of genetics · 2014Article
- Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!The Plant cell · 2014Article
- Cloning of TTG1 gene and PCR identification of genomes A, B and C in Brassica species.Genetica · 2014Article
- Genome survey sequencing provides clues into glucosinolate biosynthesis and flowering pathway evolution in allotetrapolyploid Brassica juncea.BMC genomics · 2014Article
- Differentiation of closely related genomes and chromosome identification in Brassica napus L. By simultaneous fluorescence in situ hybridization and genomic in situ hybridization.Doklady. Biochemistry and biophysics · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The two genomes (A and C) of the allopolyploid Brassica napus have been clearly distinguished using genomic in situ hybridization (GISH) despite the fact that the two extant diploids, B. rapa (A, n = 10) and B. oleracea (C, n = 9), representing the progenitor genomes, are closely related. Using DNA from B. oleracea as the probe, with B. rapa DNA and the intergenic spacer of the B. oleracea 45S rDNA as the block, hybridization occurred on 9 of the 19 chromosome pairs along the majority of their length. The pattern of hybridization confirms that the two genomes have remained distinct in B. napus line DH12075, with no significant genome homogenization and no large-scale translocations between the genomes. Fluorescence in situ hybridization (FISH)-with 45S rDNA and a BAC that hybridizes to the pericentromeric heterochromatin of several chromosomes-followed by GISH allowed identification of six chromosomes and also three chromosome groups. Our procedure was used on the B. napus cultivar Westar, which has an interstitial reciprocal translocation. Two translocated segments were detected in pollen mother cells at the pachytene stage of meiosis. Using B. oleracea chromosome-specific BACs as FISH probes followed by GISH, the chromosomes involved were confirmed to be A7 and C6.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.