ArticleThe Journal of allergy and clinical immunology2009
Interferons modulate Fc epsilon RI-dependent production of autoregulatory IL-10 by circulating human monocytoid dendritic cells.
Article in The Journal of allergy and clinical immunology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Modulation of dendritic cell innate and adaptive immune functions by oral and sublingual immunotherapy.Clinical immunology (Orlando, Fla.) · 2014Trial
- SHIP-1 Differentially Regulates IgE-Induced IL-10 and Antiviral Responses in Human Monocytes.European journal of immunology · 2025Article
- SHIP-1 differentially regulates IgE-induced IL-10 and antiviral responses in human monocytes.bioRxiv : the preprint server for biology · 2024Article
- Shrimp allergen extract immunotherapy induces prolonged immune tolerance in a gastro-food allergy mouse model.PloS one · 2024Article
- Epithelial Cell-Associated Galectin-3 Activates Human Dendritic Cell Subtypes for Pro-Inflammatory Cytokines.Frontiers in immunology · 2020Article
- Induction of Interleukin-10 Producing Dendritic Cells As a Tool to Suppress Allergen-Specific T Helper 2 Responses.Frontiers in immunology · 2018Review
- Immunoglobulin E-Mediated Autoimmunity.Frontiers in immunology · 2018Review
- Fifty years later: Emerging functions of IgE antibodies in host defense, immune regulation, and allergic diseases.The Journal of allergy and clinical immunology · 2016Review
- The role of FcεRI expressed in dendritic cells and monocytes.Cellular and molecular life sciences : CMLS · 2015Review
- IgE cross-linking critically impairs human monocyte function by blocking phagocytosis.The Journal of allergy and clinical immunology · 2013Article
- Cellular immune response parameters that influence IgE sensitization.Journal of immunological methods · 2012Review
- IgE stimulates human and mouse arterial cell apoptosis and cytokine expression and promotes atherogenesis in Apoe-/- mice.The Journal of clinical investigation · 2011Article
- Dendritic cell and T cell responses in children with food allergy.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2011Article
- Subcutaneous allergen immunotherapy restores human dendritic cell innate immune function.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundImmature human blood monocytoid dendritic cells (mDCs) express high-affinity receptors for IgE (Fc epsilon RI), yet their exact function and regulation remain poorly understood.
objectiveWe sought to characterize Fc epsilon RI-dependent cytokine responses and their regulation in circulating human blood mDCs.
methodsFc epsilon RI-dependent cytokine responses of circulating mDCs were studied by using anti-Fc epsilon RI alpha stimulation. Plasmacytoid dendritic cell (pDC) cross-regulation through Toll-like receptor 9 on these responses was investigated by examining the effects of exogenous IFN-alpha pretreatment and by coculturing pDCs and mDCs stimulated with CpG. Culture supernatants were analyzed by means of ELISA to determine cytokine levels. Cell markers were determined by means of flow cytometry.
resultsmDCs express marked levels of Fc epsilon RI (net mean fluorescence intensity, 196 +/- 49; n = 4). After Fc epsilon RI-dependent activation in mDCs, TNF-alpha (2189 +/- 864 pg/10(6) mDCs, n = 3) levels were upregulated within 4 hours, whereas IL-10 (112 +/- 47 pg/10(6) mDCs, n = 3) levels were detectable only after 24 hours of incubation. After adding IL-10-neutralizing antibody, TNF-alpha Fc epsilon RI-dependent responses were significantly augmented (3903 +/- 197 pg/10(6) mDCs, P < .01, n = 3). Conversely, recombinant IL-10 dose-dependently inhibited Fc epsilon RI-mediated TNF-alpha responses up to 86% +/- 3% (n = 3, P < .001). Pretreatment of mDCs with IFN-alpha (100 U/mL) enhanced Fc epsilon RI-dependent secretion of IL-10 by 3.2-fold (183 +/- 11 pg/10(6) mDCs, n = 4) compared with that seen in untreated cells (57 +/- 33 pg/10(6) mDCs, P < .001, n = 4). In pDC/mDC cocultures pretreated with CpG, Fc epsilon RI-dependent IL-10 secretion by mDCs was similarly augmented by 3-fold.
conclusionsAutocrine secretion of IL-10, a critical autoregulator of Fc epsilon RI-dependent proinflammatory responses in mDCs, is cross-regulated by IFN-alpha, a major product of Toll-like receptor 9 responses in pDCs that normally promotes T(H)1 immunity.
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