ArticleJournal of molecular biology2008
Structure of the cyclomodulin Cif from pathogenic Escherichia coli.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Review
- Shiga toxin-producing Escherichia coli plasmid diversity reveals virulence potential and control opportunities in animal hosts.Scientific reports · 2025Article
- The Pvc15 ATPase selectively associates effector proteins with theRoyal Society open science · 2024Article
- The Nϵ-Rule for Serine, but Not Cysteine Catalytic Triads.Angewandte Chemie (International ed. in English) · 2022Article
- Bacterial virulence mediated by orthogonal post-translational modification.Nature chemical biology · 2020Review
- CNF1-like deamidase domains: common Lego bricks among cancer-promoting immunomodulatory bacterial virulence factors.Pathogens and disease · 2018Review
- Heterogeneous Family of Cyclomodulins: Smart Weapons That Allow Bacteria to Hijack the Eukaryotic Cell Cycle and Promote Infections.Frontiers in cellular and infection microbiology · 2017Review
- Article
- Killing of Microbes and Cancer by the Immune System with Three Mammalian Pore-Forming Killer Proteins.Frontiers in immunology · 2016Review
- Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2.eLife · 2015Article
- Review
- Exploitation of the host ubiquitin system by human bacterial pathogens.Nature reviews. Microbiology · 2014Review
- Structural and enzymatic characterization of a host-specificity determinant from Salmonella.Acta crystallographica. Section D, Biological crystallography · 2014Article
- What a difference a Dalton makes: bacterial virulence factors modulate eukaryotic host cell signaling systems via deamidation.Microbiology and molecular biology reviews : MMBR · 2013Review
- The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics.The Journal of biological chemistry · 2013Article
- Solubility-based genetic screen identifies RING finger protein 126 as an E3 ligase for activation-induced cytidine deaminase.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Complex structure of OspI and Ubc13: the molecular basis of Ubc13 deamidation and convergence of bacterial and host E2 recognition.PLoS pathogens · 2013Article
- Structural mechanism of ubiquitin and NEDD8 deamidation catalyzed by bacterial effectors that induce macrophage-specific apoptosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The molecular basis of ubiquitin-like protein NEDD8 deamidation by the bacterial effector protein Cif.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida.The FEBS journal · 2011Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacterial pathogens have evolved a sophisticated arsenal of virulence factors to modulate host cell biology. Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) use a type III protein secretion system (T3SS) to inject microbial proteins into host cells. The T3SS effector cycle inhibiting factor (Cif) produced by EPEC and EHEC is able to block host eukaryotic cell-cycle progression. We present here a crystal structure of Cif, revealing it to be a divergent member of the superfamily of enzymes including cysteine proteases and acetyltransferases that share a common catalytic triad. Mutation of these conserved active site residues abolishes the ability of Cif to block cell-cycle progression. Finally, we demonstrate that irreversible cysteine protease inhibitors do not abolish the Cif cytopathic effect, suggesting that another enzymatic activity may underlie the biological activity of this virulence factor.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.