ArticleBMC microbiology2008
Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Establishment and application of a new one-step multiplex PCR for the accurate detection and differentiation of Salmonella Gallinarum biovars Pullorum and Gallinarum.BMC veterinary research · 2026Article
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- Antimicrobial Susceptibility and Frequency ofAntibiotics (Basel, Switzerland) · 2021Article
- Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes ofMicroorganisms · 2021Article
- Rapid Real-Time Polymerase Chain Reaction forFrontiers in microbiology · 2021Article
- Rapid Detection and Differentiating of the PredominantFrontiers in cellular and infection microbiology · 2021Article
- PCR-based detection and serovar identification of Salmonella in retail meat collected from wet markets in Metro Manila, Philippines.PloS one · 2020Article
- Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars.Annals of clinical microbiology and antimicrobials · 2019Article
- Designing a biochip following multiplex polymerase chain reaction for the detection of Salmonella serovars Typhimurium, Enteritidis, Infantis, Hadar, and Virchow in poultry products.Journal of food and drug analysis · 2018Article
- Molecular characterisation of Salmonella strains isolated from outbreaks and sporadic cases of diarrhoea occurred in Paraná State, South of Brazil.Epidemiology and infection · 2017Article
- An Efficient Multiplex PCR-Based Assay as a Novel Tool for Accurate Inter-Serovar Discrimination ofFrontiers in microbiology · 2017Article
- One-Step PCR Detection ofFrontiers in microbiology · 2016Article
- An allelotyping PCR for identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium.Journal of visualized experiments : JoVE · 2011Article
- Article
- The analysis of groEL gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians by PCR-Restriction Fragment Length Polymorphism method.Veterinary research communications · 2011Article
- PCR-Restriction Fragment Length Polymorphism analysis of fljB gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians.Iranian journal of microbiology · 2010Article
- Identification by PCR of non-typhoidal Salmonella enterica serovars associated with invasive infections among febrile patients in Mali.PLoS neglected tropical diseases · 2010Article
- Synergistic antibacterial and anti-inflammatory activity of temporin A and modified temporin B in vivo.PloS one · 2009Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClassical Salmonella serotyping is an expensive and time consuming process that requires implementing a battery of O and H antisera to detect 2,541 different Salmonella enterica serovars. For these reasons, we developed a rapid multiplex polymerase chain reaction (PCR)-based typing scheme to screen for the prevalent S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium.
resultsBy analyzing the nucleotide sequences of the genes for O-antigen biosynthesis including wba operon and the central variable regions of the H1 and H2 flagellin genes in Salmonella, designated PCR primers for four multiplex PCR reactions were used to detect and differentiate Salmonella serogroups A/D1, B, C1, C2, or E1; H1 antigen types i, g, m, r or z10; and H2 antigen complexes, I: 1,2; 1,5; 1,6; 1,7 or II: e,n,x; e,n,z15. Through the detection of these antigen gene allele combinations, we were able to distinguish among S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. The assays were useful in identifying Salmonella with O and H antigen gene alleles representing 43 distinct serovars. While the H2 multiplex could discriminate between unrelated H2 antigens, the PCR could not discern differences within the antigen complexes, 1,2; 1,5; 1,6; 1,7 or e,n,x; e,n,z15, requiring a final confirmatory PCR test in the final serovar reporting of S. enterica.
conclusionMultiplex PCR assays for detecting specific O and H antigen gene alleles can be a rapid and cost-effective alternative approach to classical serotyping for presumptive identification of S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium.
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